The relief of hypoxic microenvironment using an O_(2) self-sufficient fluorinated nanoplatform for enhanced photodynamic eradication of bacterial biofilms  

作　　者：Lingyun Zou Dengfeng Hu Fanjin Wang Qiao Jin Jian Ji 

机构地区：[1]MOE Key Laboratory of Macromolecule Synthesis and Functionalization of Ministry of Education,Department of Polymer Science and Engineering,Zhejiang University,Hangzhou 310027,China

出　　处：《Nano Research》2022年第2期1636-1644,共9页纳米研究（英文版）

基　　金：supported by National Key Research and Development Project(No.2020YFE0204400);National Natural Science Foundation of China(No.52022090);Zhejiang Provincial Ten Thousand Talents Program(No.2018R52001).

摘　　要：Hypoxia,an important characteristic of bacterial biofilms,can hinder the generation of reactive oxygen species(ROS)in photodynamic therapy(PDT),leading to reduced therapeutic efficacy of PDT.In order to address this issue,fluorinated liposome was fabricated as an oxygen-sufficient nanoplatform for enhanced photodynamic eradication of bacterial biofilms.The liposomes(denoted as Lip-Ce6-PFH@O_(2))were prepared by co-encapsulation of O_(2) carrier perfluorohexane(PFH)and photosensitizer chlorin e6(Ce_(6)).Lip-Ce6-PFH@O_(2) could achieve efficient biofilm penetration due to the positively charged surface.The hypoxic microenvironment of biofilms would then be relieved,leading to the generation of more ROS under laser irradiation.Therefore,the bactericidal capability of PDT could be significantly improved because of the co-delivered O_(2) carrier PFH.Lip-Ce6-PFH@O_(2) exhibited much better antibiofilm ability than that of Lip-Ce6 both in vitro and in vivo.Meanwhile,Lip-Ce6-PFH@O_(2) also effectively alleviated inflammation symptoms and accelerated wound healing in the mice model.In general,this study provides a new paradigm to enhance the therapeutic efficacy of PDT for efficient biofilm eradication.

关 键 词：biofilm hypoxia perfluorohexane LIPOSOME photodynamic therapy 

分 类 号：R73[医药卫生—肿瘤]