超声波辅助提取金枪鱼蒸煮液鱼油工艺研究及品质分析  

Research on ultrasonic-assisted extraction technology and quality analysis of fish oil from Zhoushan tuna cooking liquor

作　　者：王新宇 王欣欣 位正鹏 陈奕名 王鹏[1] 李银平 乔乐克 刘瑞志[2] WANG Xinyu;WANG Xinxin;WEI Zhengpeng;CHEN Yiming;WANG Peng;LI Yinping;QIAO Leke;LIU Ruizhi(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;Chinese Research Academy of Environmental Sciences,Beijing 100012,China;College of Marine Science and Biological Engineering,Qingdao University of Science and Technology,Qingdao 266042,China;National and Local Joint Engineering Laboratory for Marine Functional Food,Rongcheng 264309,China)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]中国环境科学研究院,北京100012 [3]青岛科技大学海洋科学与生物工程学院,山东青岛266042 [4]海洋功能食品国家地方联合工程实验室,山东荣成264309

出　　处：《水产科技情报》2022年第2期82-90,共9页Fisheries Science & Technology Information

基　　金：舟山市长江生态环境保护修复驻点跟踪研究项目(SZGXZS2020068)。

摘　　要：为探索金枪鱼蒸煮液鱼油高值化开发利用技术,对超声辅助乙醇提取金枪鱼蒸煮液鱼油工艺进行优化,通过单因素试验研究液料比、温度、时间和功率对鱼油提取率的影响,在此基础上采用响应面法进一步优化工艺条件。试验结果表明,最优工艺条件为:提取温度34℃,提取时长60 min,超声功率154 W。该工艺条件下鱼油的提取率为91.32%。在无精制过程条件下,采用该工艺提取的鱼油,其不溶性杂质、酸价、碘价、过氧化值可达精制鱼油标准,且铅、铬、镉、无机砷、甲基汞、多氯联苯均在限量范围内。采用该工艺提取的鱼油富含ω-3多不饱和脂肪酸,其中DHA和EPA总量达40.49%。提取的鱼油对DPPH自由基的半抑制质量浓度(IC_(50))为6.13 mg/mL,且低浓度鱼油可与维生素E或绿原酸组合产生协同抗氧化作用。Cooking is an important link in the production and processing of tuna. In order to explore the high-value development and utilization technology of tuna liquid cooking fish oil and optimize the ultrasonic assisted ethanol extraction process, the effects of liquid-to-material ratio, temperature, time and power on the extraction rate of fish oil were studied by single factor experiment, and the response surface methodology was used to further optimize the process conditions. The results showed that the optimal process conditions were as follows: extraction temperature 34 ℃, extraction time 60 min, ultrasonic power 154 W. Under these conditions, the maximum extraction rate of fish oil was 91.32%. The insoluble impurities, acid value, iodine value, and peroxide value of fish oil extracted by this process without refining can reach the standard of refined fish oil, and lead, chromium, cadmium, inorganic arsenic, methylmercury and polychlorinated biphenyls are all within limited range. The results of fatty acid composition showed that the fish oil was rich in omega-3 polyunsaturated fatty acids, and the total amount of DHA and EPA reached 40.49%. The half-inhibitory mass concentration(IC_(50)) of fish oil on DPPH free radicals was 6.13 mg/mL, and low-concentration fish oil could combine with vitamin E or chlorogenic acid to produce a synergistic antioxidant effect. The research results can provide scientific basis for the development of tuna cooking liquid fish oil functional food.

关 键 词：金枪鱼 蒸煮液 提取 超声波辅助 Ω-3多不饱和脂肪酸 抗氧化 

分 类 号：TS225.24[轻工技术与工程—粮食、油脂及植物蛋白工程]