蛹虫草发酵产虫草素工艺优化  

Optimization of cordycepin production from Cordyceps militaris

作　　者：唐成伦 王松涛 赵南 邹亚男 陈勇[3] 刘庆国 刘淼[2] TANG Chenglun;WANG Songtao;ZHAN Nan;ZOU Yanan;CHEN Yong;LIU Qingguo;LIU Miao(Nanjing Hi-tech Biological Technology Research Institute Co.Ltd.,Nanjing 210032,China;Luzhou Laojiao Co.Ltd.,Luzhou 646000,China;College of Biotechnology and Pharmaceutical Engineering Nanjing University of Technology,Nanjing 210032,China)

机构地区：[1]南京高新工大生物技术研究院有限公司,江苏南京210032 [2]泸州老窖股份有限公司,四川泸州646000 [3]南京工业大学生物与制药工程学院,江苏南京210032

出　　处：《食品与发酵工业》2022年第5期106-110,共5页Food and Fermentation Industries

摘　　要：为确定蛹虫草产虫草素放大工艺条件,考察了菌种培养质量和发酵高径比对虫草素合成的影响。确定种子培养最佳条件为:转速250 r/min,接种量15%,扩培级数2次,此条件下虫草素发酵产量可达6 g/L左右。另外,装液量最适高径比为2 cm/30 cm。在此条件下,进行了120 L多层反应器发酵工艺验证。发酵25 d,虫草素产量为5.32 g/L,转化率57%,与小试工艺结果基本一致。实验结果表明,工艺放大具有应用可行性,为进一步工业化生产提供了参考。In order to determine the amplification technology for cordycepin, the effects of cultivation quality and height-diameter ratio of fermentation on the synthesis of cordycepin were investigated. The results showed that the concentration of cordycepin reached 6 g/L when the optimal conditions of seed cultivation were as follows: the rotation speed of 250 r/min, the inoculum size of 15%, and the subculture times of two. In addition, the optimal height-diameter ratio of liquid loading was 2 cm/30 cm. Under these conditions, a 120 L multi-layer reactor was used to verify the fermentation process. After 25 days of fermentation, the concentration of cordycepin was 5.32 g/L and the conversion rate was 57%, which was consistent with the results of small scale test. The experimental results showed that the process scaling up is feasible, which provides a reference for further industrialization.

关 键 词：蛹虫草 虫草素 工艺放大 多层反应器 高径比 

分 类 号：R284[医药卫生—中药学]