杜仲籽油对2型糖尿病KKAy小鼠糖脂代谢及肠道菌群的影响  

Effects of Eucommia ulmoides seed oil on glycolipid metabolism and gut microbiota in KKAy mice with type 2 diabetes mellitus

作　　者：武毅楠 高梦珂 李攀峰 薛柯 李钦[1] WU Yi-Nan;GAO Meng-Ke;LI Pan-Feng;XUE Ke;LI Qin(College of Pharmacy,Henan University,Kaifeng 475004,China)

机构地区：[1]河南大学药学院,开封475004

出　　处：《食品安全质量检测学报》2022年第3期728-736,共9页Journal of Food Safety and Quality

基　　金：国家重点研发计划项目(2017YFD0600702)。

摘　　要：目的探讨杜仲籽油对2型糖尿病KKAy小鼠糖脂代谢及肠道菌群的影响。方法24只KKAy小鼠随机分为3组(模型组、阳性药组、杜仲籽油组),8只C57BL/6J小鼠为正常组。实验7周后测定小鼠体重、空腹血糖和空腹胰岛素、糖耐受量、血脂四项和肝功能两项,进行肝脏油红O染色和小鼠肠道菌群检测。结果与模型组相比,杜仲籽油组降低小鼠空腹血糖、空腹胰岛素、糖耐量曲线下面积、胆固醇和甘油三酯、低密度脂蛋白含量(P<0.05),减轻肝脏脂肪变性,改善肠道菌群失调,提高微生物多样性,降低厚壁菌门(Firmicutes)/拟杆菌门(Bacteroidetes)比例,升高布劳特氏菌属(Blautia)和乳杆菌属(Lactobacillus)相对丰度(P<0.05),有降低嗜胆菌属(Bilophila)和厌氧棒状菌属(Anaerostipes)相对丰度的趋势(P>0.05)。结论杜仲籽油对高脂饮食引起的KKAy小鼠糖脂代谢及肠道菌群紊乱有一定的调节作用。Objective To evaluate the effects of Eucommia ulmoides seed oil on glycolipid metabolism and gut microbiota in KKAy mice with type 2 diabetes mellitus.Methods 24 KKAy mice were randomly divided into 3 groups(model group,positive drug group,Eucommia ulmoides seed oil group),and 8 C57 BL/6 J mice were normal group.After 7 weeks of the experiment,the body weight,fasting blood glucose and fasting insulin,glucose tolerance,4 items of blood lipids and 2 items of liver function were measured,and the liver oil red O staining and the intestinal flora of the mice were detected.Results Compared with the model group,Eucommia ulmoides seed oil decreased the content of fasting blood glucose,fasting insulin,glucose tolerance curve area,total cholesterol,total triglyceride and low-density lipoprotein(P<0.05),alleviated liver steatosis,improved intestinal flora imbalance,increased microbial diversity,decreased the ratio of Firmicutes/Bacteroidetes,and increased the relative abundance of Blautia and Lactobacillus(P<0.05).There was a tendency to decrease the relative abundance of Cholangiophil(Bilophila)and anaerobic Corynebacterium(Anaerostipes)(P>0.05).Conclusion Eucommia ulmoides seed oil has a certain regulatory effect on glycolipid metabolism and gut microbiota disorder in KKAy mice fed high fat diet.

关 键 词：KKAy 杜仲籽油 糖脂代谢 肠道菌群 

分 类 号：R285.5[医药卫生—中药学]