淀粉中直链淀粉含量检测技术的研究进展  

Research progress of amylose determination technology in starch

作　　者：丁振 冯朵 曹盼盼 李恩鹏 李松南 DING Zhen;FENG Duo;CAO Panpan;LI Enpeng;LI Songnan(College of Agriculture, Yangzhou University, Yangzhou, Jiangsu 225009, China;Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, China;Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu 225009, China)

机构地区：[1]扬州大学农学院,江苏扬州225009 [2]扬州大学教育部农业与农产品安全国际合作联合实验室,江苏扬州225009 [3]扬州大学农业科技发展研究院,江苏扬州225009

出　　处：《美食研究》2022年第1期87-94,共8页Journal of Researches on Dietetic Science and Culture

基　　金：扬州大学高层次人才科研启动基金项目(137012153);安徽农业大学茶树生物学与资源利用国家重点实验室开放基金资助项目(SKLTOF20210114)。

摘　　要：直链淀粉是淀粉的重要组成部分,其含量也是衡量淀粉的糊化、流变、老化和消化等功能性质的重要指标之一。直链淀粉含量的准确测定,对淀粉类制品的精深加工和高效应用有着重要的意义。基于直链淀粉的结构和性质,碘染法、光谱法、色谱法和热力学法等技术被应用于直链淀粉含量的检测中,并正在进一步朝着快速、方便、准确和灵敏的方向发展。目前的直链淀粉检测技术众多,还需根据其工作原理、测试场景和可及的设备与试剂,“因地制宜”地选择合适的检测技术,且在测试过程尽可能减少其他因素的干扰,才能得到精确度高、准确度高和重复性好的结果。Amylose is an important component of starch,its content is also one of the important indexes to measure the functional properties of starch,such as gelatinization,rheology,retrogradation,and digestion.The accurate determination of amylose content is of great significance to the intensive processing and efficient application of starch-based products.Based on the structure and properties of amylose,iodine staining,spectroscopy,chromatography,and thermodynamics have been applied to the determination of amylose content,and are further developed towards the direction of fast speed,convenience,accuracy and sensitivity.There are many amylose determination technologies at present,therefore it is necessary to select the appropriate detection technology according to its working principle,test condition and accessible equipment and reagents,to reduce the interference factors as much as possible and obtain the results with high accuracy and good repeatability.

关 键 词：直链淀粉 含量检测 碘染法 光谱法 色谱法 热力学法 

分 类 号：TS972.111[轻工技术与工程]