振动胁迫对双孢蘑菇活性氧代谢和抗氧化性能的影响  

Effects of Vibration Stress on Active Oxygen Metabolism and Antioxidant Properties of Agaricus Bisporus

作　　者：孙炳新[1] 于官楚 任浩 唐建新 SUN Bingxin;YU Guanchu;REN Hao;TANG Jianxin(College of Food Science,Shenyang Agricultural University,Shenyang 110866,China)

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110866

出　　处：《包装学报》2022年第1期17-25,共9页Packaging Journal

基　　金：辽宁省科技厅揭榜挂帅科技攻关专项课题(2021JH1/10400035)。

摘　　要：以新鲜的双孢蘑菇为原材料,研究了3 Hz振动频率下经过1,2 h的振动处理(以未经振动的双孢蘑菇作对照)对双孢蘑菇色泽、活性氧代谢和抗氧化性能的影响。结果表明:振动胁迫加速了双孢蘑菇超氧阴离子(O_(2)^(-))的产生和过氧化氢(H_(2)O_(2))含量的积累,不同振动时间下双孢蘑菇产生的O_(2)^(-)活力是对照组的2.89倍(1 h)和3.31倍(2 h),H_(2)O_(2)含量是对照组的1.38倍(1 h)和1.56倍(2 h);振动胁迫诱导超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)等抗氧化酶活性迅速升高,相对电导率、丙二醛(MDA)含量大幅上升,抗坏血酸加速分解,总酚、类黄酮含量和多酚氧化酶(PPO)酶活性峰值提前出现。振动胁迫后的双孢蘑菇活性氧自由基大量积累,细胞膜氧化损伤加剧,最终加速了双孢蘑菇的褐变。Taking Agaricus bisporus as the raw material,the effects of 1 h and 2 h vibration treatments(with unvibrated mushrooms as a control)on the color,active oxygen metabolism and antioxidant properties of fresh Agaricus bisporus under 3 Hz vibration frequency were studied.The results showed that vibration stress accelerated the production of O_(2)^(-) and the accumulation of H_(2)O_(2) content,the O_(2)^(-) production of the Agaricus bisporus treated with different vibration time was 2.89 times(1 h)and 3.31 times(2 h)of the control group,H_(2)O_(2) content was 1.38 times(1 h)and 1.56 times(2 h)of the control group.After vibration stress,SOD,CAT and APX antioxidant enzyme activities,relative conductivity and MDA content increased significantly.In addition,ascorbic acid decomposition accelerated,total phenols,flavonoids content and PPO enzyme activity peaks appeared earlier.Due to the large accumulation of active oxygen free radicals,the oxidative damage of the cell membrane was aggravated and eventually accelerated the browning of the mushrooms.

关 键 词：双孢蘑菇 振动胁迫 活性氧代谢 抗氧化性能 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]