Experimental study on the vitrification and xenotransplantation of human ovarian tissue  

作　　者：Jinghui Zhao Yuping Ge Jingyi Li Yucui Zeng Guoyi Dong Liping Zeng Juanjuan Dai Hui Du Mumin Shao Xuewen Yu Huan Li Fang He Zhouchun Shang Chun Wang G
abor Vajt Huiru Tang Ruifang Wu Yutao Du 

机构地区：[1]Department of Obstetrics and Gynecology,Peking University Shenzhen Hospital,518036,China [2]BGI-Shenzhen,Shenzhen,518083,China [3]Department of Obstetrics and Gynecology,Shantou University Medical college,515041,China [4]Department of Pathology,Shenzhen Affiliated Hospital,Guangzhou University of Chinese Medicine,Shenzhen,510006,China

出　　处：《Gynecology and Obstetrics Clinical Medicine》2022年第1期38-42,共5页妇产科临床医学（英文）

基　　金：the Science and Technology Development Fund Project of Shenzhen,China(JCYJ 20140415162338852);Scientific Research Project of Shenzhen health family planning,China(201401038);Natural Science Foundation of Guangdong Province,China(2015A030313889).

摘　　要：Background:Cryopreservation of ovarian tissue is a promising method for preserving fertility.Transmission electron microscopy(TEM)is an evaluation system for cryo-injury during the cooling and warming process which is very laborious and needs to be optimized.Objective:In this study,we evaluated that serum 17β-oestradiol(E2)may be used as an indicator of vitrified ovarian tissue.Methods:Immunodeficient nude mice were used as hosts for xenografting of vitrified-warmed human ovarian tissues.A total of 54 mice were divided into two group:vitrified ovarian xenotransplant(VOX)group(n?45)and non-transplant control group(n?9).The transplanted mice were grouped into vitrified/warmed grafted-4 weeks(VOX-4w,n?15),vitrified/warmed grafted-6weeks(VOX-6w n?15)and vitrified/warmed grafted-12 weeks(VOX-12w n?15)according to the time after transplantation.The viable and functional recovery of grafted ovarian tissue was assessed by light microscopy,transmission electron microscopy,and hormone(E2)assays.Results:Serum E2 concentration was significantly higher in VOX-6w(group(21.07 pg/ml)than that of VOX-12w group(15.59 pg/ml).VOX-12w group showed a lower value(12.61 pg/ml)for E2 concentration.The trend for E2 concentration was consistent with the morphological identification of the grafts.Conclusion:In vivo serum hormone E2 released by cortical biopsies can be used as a functional marker for xenotransplanted vitrified-warmed human ovarian tissue reserve.

关 键 词：Vitrification-warmed Ovarian tissue 17β-oestradiol XENOTRANSPLANTATION 

分 类 号：R73[医药卫生—肿瘤]