香兰素的铜掺杂碳量子点的荧光法检测  

Fluorescence Detection of Vanillin by Copper Doped Carbon Quantum Dots

作　　者：司晓晶 邓卢辰 徐鑫 李丽[1] 宫霞[1] SI Xiaojing;DENG Luchen;XU Xin;LI Li;GONG Xia(Department of Food Science,Shanghai Business School,Shanghai 200235,China;College of Qian Weichang,Shanghai University,Shanghai 200444,China)

机构地区：[1]上海商学院食品系,上海200235 [2]上海大学钱伟长学院,上海200444

出　　处：《食品工业科技》2022年第7期280-285,共6页Science and Technology of Food Industry

基　　金：上海市属高校应用型本科试点专业建设项目(第六批);上海市大学生创新创业训练计划项目S202112050127。

摘　　要：目的:建立一种基于铜掺杂碳量子点(Cu-CDs)的荧光检测方法用于食品中香兰素含量的分析。方法:以抗坏血酸作为碳源,乙酸铜作为金属掺杂剂,通过一步水浴合成法制备性能稳定的Cu-CDs,通过X-射线能谱、红外、紫外、荧光等技术对制备的Cu-CDs进行形貌、元素及光学性能的表征,并研究该碳点与香兰素的猝灭作用。结果:Cu-CDs溶液中C、Cu分布均匀,表面富含氨基、羧基、羟基等官能团,说明其具有很好的水溶性。在pH7.0的磷酸盐缓冲溶液中,香兰素对Cu-CDs有强荧光猝灭作用。当香兰素浓度在1~400μmol/L时,与其对应荧光强度的对数值与呈良好线性关系,线性方程为y=0.0045x+0.1934(R2=0.9917),检测限为0.33μmol/L(S/N=3),并应用于香草精样品中香兰素含量测定,加标回收率为98%~110%。结论:Cu-CDs荧光法测定香兰素的方法成本低、专属性强且操作简便,具有较好的应用前景。Objective:A fluorescence detection method based on copper doped carbon quantum dots(Cu-CDs)was developed for the determination of vanillin in food.Method:Cu-CDs,with stable performance,were prepared by one-step hydrothermal method using ascorbic acid as precursors and copper sulfate as metal dopant.The morphology,elementary composition and optical properties of the synthesized Cu-CDs were characterized by energy dispersive X-ray spectrometers,fourier transform infrared spectroscopy,ultraviolet and fluorescence techniques.The quenching effect of the Cu-CDs with vanillin was further studied.Results:C and Cu were evenly distributed in Cu-CDs solution.The Cu-CDs were rich in functional groups such as amino,carboxyl and hydroxyl groups,indicating that Cu-CDs had good water solubility.It was also found that vanillin had strong fluorescence quenching effect on Cu-CDs in the pH7.0 KH2PO4-K2HPO4 buffer solution,and the concentration of tetracycline in the range of 1~400μmol/L showed a good linear relationship with the fluorescence quenching intensity of Cu-CDs,and the linear equation was y=0.0045x+0.1934(R2=0.9917),the detection limit was 0.33μmol/L.What’s more,it was applied to the determination of vanillin in food samples,the recovery was 98%~110%.Conclution:The method for the determination of vanillin has the advantages of low cost,high specificity,simple operation,and a good application prospect.

关 键 词：铜掺杂碳量子点 水热法 荧光猝灭 香兰素 

分 类 号：O657.39[理学—分析化学]