Perturbations of the ileal mycobiota by necrotic enteritis in broiler chickens  

作　　者：Qing Yang Jing Liu Kelsy J.Robinson Melanie A.Whitmore Sydney N.Stewart Guolong Zhang 

机构地区：[1]Department of Animal and Food Sciences,Oklahoma State University,Stillwater,OK,USA [2]Poultry Production and Product Safety Research Unit,USDA-Agricultural Research Service,Fayetteville,AR,USA [3]Safety and Security Division,Institute for Public Research,CNA,Arlington,VA,USA

出　　处：《Journal of Animal Science and Biotechnology》2022年第2期520-535,共16页畜牧与生物技术杂志（英文版）

基　　金：supported by the USDA National Institute of Food and Agriculture (grant no. 2018–68003-27462 and 2018–67011-28041);the Ralph F. and Leila W. Boulware Endowment Fund;Oklahoma Agricultural Experiment Station Project H-3112。

摘　　要：Background:Intestinal microbiota is critical for maintaining animal health and homeostasis.However,involvement of the fungal community,also known as the mycobiota,in animal health and disease is poorly understood.This study was aimed to examine the association between the intestinal mycobiota and the severity of necrotic enteritis(NE),an economically significant poultry disease.Methods:A total of 90 day-of-hatch Cobb broilers were infected with Eimeria maxima on d 10,followed by an oral challenge with C.perfringens on d 14 to induce NE,while another 10 broilers were served as mock-infected controls.On d 17,the lesions in the jejunum were scored,and the ileal digesta were subjected to DNA isolation and real-time PCR quantification of total bacterial and fungi populations.Internal transcribed spacer 2(ITS2)amplicon sequencing was also performed to profile the ileal mycobiota composition.Changes in the ileal mycobiota in response to NE were investigated.Spearman correlation analysis was further conducted to identify the correlations between relative abundances of individual ileal fungi and the severity of NE.Results:While the total bacterial population in the ileum was increased by 2-to 3-fold in NE chickens,the total fungal population was progressively declined in more exacerbated NE,with the most severely infected chickens showing a nearly 50-fold reduction relative to mock-infected controls.Richness of the ileal mycobiota also tended to reduce in chickens with NE(P=0.06).Compositionally,among 30 most abundant fungal amplicon sequence variants(ASVs),11 were diminished and 7 were enriched(P<0.05),while 12 remained largely unchanged in NEafflicted chickens(P>0.05).Multiple Wallemia and Aspergillus species were markedly diminished in NE(P<0.05)and also showed a significant negative correlation with NE severity(P<0.05).Conclusions:Dysbiosis of the ileal mycobiota is induced evidently by NE and the extent of the dysbiosis is positively correlated with disease severity.These findings suggest a possible role of the intes

关 键 词：Antimicrobial resistance C.perfringens DYSBIOSIS ITS sequencing MICROBIOME MYCOBIOTA Necrotic enteritis POULTRY 

分 类 号：S858.31[农业科学—临床兽医学]