Determination of 3-MeO-PCP in human blood and urine in a fatal intoxication case,with a specific focus on metabolites identification  

作　　者：Nadia Arbouche Pascal Kintz Cecile Zagdoun Laurie Gheddar Jean-Sébastien Raul Alice Ameline 

机构地区：[1]ainstitut de Médecine Légale,strasbourg,France [2]X-Pertise consulting,Mittelhausbergen,France [3]UnitéMédico-Judiciaire,hôpital emile Muller,Mulhouse,France

出　　处：《Forensic Sciences Research》2021年第4期208-214,共7页法庭科学研究（英文）

摘　　要：3-Methoxyphencyclidine(3-MeO-PCP)is a new psychoactive substance that belongs to the phencyclidines family,first identified in Europe in 2012.This drug presents a stronger binding to N-methyl-D-aspartate(NMDA)receptors when compared to phencyclidine,which results in more potent effects,even at low concentrations.Very few articles have been published regarding 3-MeO-PCP in forensic toxicology.In this paper,the authors present a fatal 3-MeO-PCP intoxication case.In addition to the detection of the parent drug,metabolites were investigated in urine and,for the first time in the scientific literature,in blood.3-MeO-PCP and its metabolites were quantitated by liquid chromatography-tandem mass spectrometry system(LC-MS/MS).Identification was confirmed by liquid chromatography-high resolution mass spectrometry(LC-HRMS).3-MeO-PCP tested positive in femoral blood(3525 ng/mL)and urine(7384 ng/mL).The femoral blood concentration was higher than the fatal concentrations range already reported in the literature(from 50 to 3200 ng/mL).3-MeO-PCP metabolites,including O-demethyl-3-MeO-PCP,piperidine-OH-3-MeO-PCP,O-demethyl-piperidine-di-OH-3-MeO-PCP and piperidine-di-OH-3-MeO-PCP,were detected in blood.In addition,two new metabolites,O-demethyl-piperidine-OH-3-MeO-PCP and O-demethyl-cyclohexyl-OH,were identified in both blood and urine.Unfortunately,due to the lack of reference material on the market,it was not possible to measure the concentration of these metabolites.However,the ratios between the metabolites and the parent drug were useful to estimate their analytical response and prevalence.At this time,considering the low ratios(<1)between metabolites and parent drug,metabolites testing does not seem useful to increase the detection window of the drug.

关 键 词：Forensic sciences forensic toxicology 3-MeO-PCP METABOLITES fatal intoxication BLOOD URINE 

分 类 号：D919.2[医药卫生—法医学]