生物发酵菊叶薯蓣富集薯蓣皂苷的研究  

Enriched dioscin from Dioscorea composita Hemsl.by biological fermentation

作　　者：崔乃菠 马境谦 余河水 李正 何永志 别松涛 CUI Nai-bo;MA Jing-qian;YU He-shui;LI Zheng;HE Yong-zhi;BIE Song-tao(School of Pharmaceutical Engineering of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617;State Key Laboratory of Components of Traditional Chinese Medicine,Tianjin 301617;Municipal Experimental Teaching Demonstration Center of Traditional Chinese Medicine Pharmaceutical Engineering,Tianjin 301617)

机构地区：[1]天津中医药大学中药制药工程学院,天津301617 [2]组分中药国家重点实验室,天津301617 [3]中药制药工程市级实验教学示范中心,天津301617

出　　处：《中南药学》2022年第2期340-344,共5页Central South Pharmacy

摘　　要：目的通过响应面分析法优化菊叶薯蓣的发酵条件,富集菊叶薯蓣中的薯蓣皂苷。方法采用响应面分析法,设计三因素三水平实验,考察发酵时间、发酵温度、接种量对菊叶薯蓣中薯蓣皂苷含量的影响。采用HPLC-ELSD测定薯蓣皂苷的含量。结果回归模型分析表明二次回归方程能够用于菊叶薯蓣生物发酵条件的优化(R^(2)=0.9685),最佳发酵条件为发酵时间47 h、发酵温度33.4℃、接种量16.3%。结论响应面分析法能够优化菊叶薯蓣的发酵条件并实现薯蓣皂苷的富集,为菊叶薯蓣的进一步研究与利用提供参考。Objective To achieve the enrichment of dioscin by optimizing the fermentation conditions of Dioscorea composita Hemsl.Methods Response-surface analysis was used to determine the fermentation efficiency.The experimental design involved the three variables:fermentation time,fermentation temperature and inoculum amount.The content of dioscin was determined by HPLCELSD.Results The mathematical-regression model demonstrated that a quadratic polynomial model could be used to optimize the fermentation conditions(R^(2)=0.9685).The optimal conditions were fermentation for 47 h,fermentation temperature at 33.4℃,and inoculum concentration at 16.3%.Conclusion The response surface analysis is an appropriate approach for the optimization of fermentation conditions for dioscin enrichment,which provides reference for the further research and utilization of Dioscorea composita Hemsl..

关 键 词：菊叶薯蓣 酵母菌 薯蓣皂苷 最佳发酵条件 响应面分析法 

分 类 号：R284[医药卫生—中药学]