环保试剂聚羟基丙烯酸和Van-clear用于HE染色效果分析  

Application of environmental protection reagents polyhydroxyacrylic acid and Van-clear in HE dyeing

作　　者：张淑艳[1] 康林[1] 张秀智[1] 徐明堂[1] 马晓丽[1] ZHANG Shuyan;KANG Lin;ZHANG Xiuzhi;XU Mingtang;MA Xiaoli(Pathology Department,Hebei Provincial People's Hospital,Shijiazhuang,Hebei 050051,China)

机构地区：[1]河北省人民医院病理科,河北石家庄050051

出　　处：《安徽医药》2022年第4期809-811,I0003,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探讨聚羟基丙烯酸和Van-clear在苏木精-伊红染色法(HE染色)中的应用效果。方法收集河北省人民医院2018年1月至2019年1月期间送检的脑组织(12例)、脾组织(18例)、骨髓组织(22例)、淋巴结组织(50例)、淋巴瘤组织(10例)、乳腺纤维腺瘤组织(62例)、宫颈腺癌组织(26例)、子宫平滑肌瘤组织(74例)、慢性宫颈炎组织(102例)、胎盘组织(35例)、脂肪组织(57例),同一部位各取材两块,采用抽签法随机分为A组和B组。A组采用传统甲醛和二甲苯处理HE切片468张,B组采用环保试剂聚羟基丙烯酸和Van-clear处理HE切片468张,观察对比两组切片HE染色情况。结果两组切片均取材完整、厚度适宜、未见褶皱或刀痕、HE染色核浆清晰、红蓝适度、透明洁净、封裱美观;A组优良率与B组优良率相比差异无统计学意义(98.08%比98.71%)(P>0.05);将A组HE染色结果作为参照,B组切片的优、良、中、差染色结果与A组染色结果的符合率分别为97.4%(456/468)、98.2%(459/468)、99.4%(465/468)、100%(468/468),本研究符合率最小为97.4%,将A组HE染色结果作为参照,B组HE染色结果预期符合率较高。结论聚羟基丙烯酸和Van-clear处理切片相较于甲醛和二甲苯更加环保、对病理工作人员的刺激性小、安全性高,在不改变任何处理程序的情况下效果相当,对于病理实验室而言具有运用推广意义。Objective To investigate the application effect of environmental protection reagents polyhydroxyacrylic acid and Van-clear in Hematoxylin eosin staining(HE staining).Methods Brain tissue(12 cases),spleen tissue(18 cases),bone marrow tissue(22 cases),lymph node tissue(50 cases),lymphoma tissue(10 cases),breast fibroma tissue(62 cases),cervical adenocarcinoma tissue(26 cases),uterine leiomyoma tissue(74 cases),chronic cervicitis tissue(102 cases),placenta tissue(35 cases)and Adipose tissue(57 cas⁃es)in Hebei Provincial People's Hospital from January 2018 to January 2019 were collected.Two pieces were taken from the same part.The pieces were assigned into group A and group B by lottery.In group A,468 HE slices were treated with traditional formalde⁃hyde and xylene,and in group B,468 HE slices were treated with polyhydroxyacrylic acid and Van-clear.The HE staining of the two groups was observed and compared.Results Two groups of slices were taken intact,suitable thickness,no wrinkles or knife marks,clear HE staining pulp,moderate red and blue,transparent and clean,and beautiful mounting.There was no significant difference in the excellent and good rate between group A and group B(98.08%vs.98.71%)(P>0.05).The HE staining results of group A were taken as a reference,and the Excellent,Good,Medium and Poor staining results of group B were 97.4%(456/468),98.2%(459/468),99.4%(465/468)and 100%(468/468)respectively in accordance with the staining results of group A.The minimum coincidence rate of this study was 97.4%.It was concluded that the expected coincidence rate of HE staining results in group A was higher than that in group B.Conclusion Compared with formaldehyde and xylene,polyhydroxyacrylic acid and Van-clear are more environmentally friendly,less irritating and safe for pathological workers.They have the same effect without changing any treatment procedures.They are of great sig⁃nificance for the application and popularization of pathological laboratories.

关 键 词：着色剂 染色与标记 丙烯酸树脂类 环保试剂 聚羟基丙烯酸 Van-clear 苏木精-伊红染色法 应用效果 

分 类 号：R-33[医药卫生]