γ-PGA发酵生产及提取工艺优化  

Optimization of fermentation and extraction processes ofγ-PGA

作　　者：冯世红 张宗华 王飞 张晓勇 杨晓芳 高帆 李颖 FENG Shihong;ZHANG Zonghua;WANG Fei;ZHANG Xiaoyong;YANG Xiaofang;GAO Fan;LI Ying(Xinjiang Amino Acid Key Technology Research and Application Innovation Team,Urumqi 831302,China;Xinjiang Engineering Research Center of Microbial Polysaccharides,Urumqi 831302,China;Xinjiang Fufeng Biotechnology Co.,Ltd.,Urumqi 831302,China)

机构地区：[1]新疆氨基酸关键技术研究与应用创新团队,新疆乌鲁木齐831302 [2]新疆微生物多糖工程技术研究中心,新疆乌鲁木齐831302 [3]新疆阜丰生物科技有限公司,新疆乌鲁木齐831302

出　　处：《发酵科技通讯》2022年第1期1-5,共5页Bulletin of Fermentation Science and Technology

基　　金：新疆维吾尔自治区科技厅科技计划研发项目(2022129886)。

摘　　要：γ-聚谷氨酸(γ-Polyglutamic acid,简称γ-PGA)是一种具有生物相容性、生物可降解性和无毒无污染的高分子聚合物。微生物发酵法生产γ-PGA工艺简单、产量较高,是γ-PGA的主要生产方法,但是γ-PGA发酵液成分复杂、黏度高,从发酵液中分离纯化γ-PGA存在一定难度。该工艺以枯草芽孢杆菌为发酵菌株,以谷氨酸钠为原料进行发酵生产γ-聚谷氨酸、优化发酵及提取工艺。实验结果表明:当发酵温度为32℃时,γ-PGA产量较高;当发酵液中谷氨酸钠质量分数为4.2%时,发酵液黏度可达到最高;采用乙醇入料提纯方式可有效去除发酵液中的色素、大分子蛋白和其他有机杂质。Gama-Polyglutamic acid(γ-PGA)is a kind of high molecular polymer with biocompatible,biodegradable,non-toxic,and pollution-free.The microbial fermentation method becomes the main way forγ-PGA production owing to its simple and high yield.However,due to the complex composition and high viscosity ofγ-PGA fermentation broth,separation and purification ofγ-PGA from the fermentation broth is difficult.In this study,γ-PGA was produced by Bacillus subtilis with glutamate sodium as raw material.The fermentation and extraction processes were optimized.The results showed that the yield ofγ-PGA was higher when the fermentation temperature was 32℃.The viscosity of fermentation broth reached the highest when glutamate content was 4.2%.Adding ethanol to purify fermentation broth can effectively remove pigments,macromolecular proteins,and other organic impurities.

关 键 词：枯草芽孢杆菌 谷氨酸钠 Γ-聚谷氨酸 工艺优化 

分 类 号：Q517[生物学—生物化学]