Capture-Release Strategy Facilitates Rapid Enzymatic Assembly of Oligosaccharides  

作　　者：Wenyuan Fang Kan Zhong Jiansong Cheng Xian-Wei Liu Chang-Cheng Liu Zhongfu Wang Hongzhi Cao 

机构地区：[1]National Glycoengineering Research Center,NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine,and Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology,Shandong University,Qingdao,Shandong 266237,China [2]Key Laboratory of Marine Drugs of Ministry of Education,Shandong Key Laboratory of Glycoscience and Glycotechnoiogy,School of Medicine and Pharmacy,Ocean University of China,Qingdao,Shandong 266003,China [3]College of Pharmacy,Nonkoi University,Tianjin 300071,China [4]Glycobiology and Glycotechnology Research Center,College of Food Science and Technology,Northwest University,Xi'an,Shaanxi 710069,China [5]Laboratory for Marine Drugs and Bioproducts,Pilot Notional Laboratory for Marine Science and Technology(Qingdao),Qingdao,Shandong 266237,China

出　　处：《Chinese Journal of Chemistry》2022年第3期343-350,共8页中国化学（英文版）

基　　金：This project was supported by the National Key Research and Development Program of China(Nos.2021YFC2101500,2018YFA0902002);the National Natural Science Foundation of China(Nos.21877073,21807064,21778035,and 21961142016);Department of Science and Technology of Shandong Province(No.2020CXGC010601);the Tianjin Natural Science Foundation(No.19JCYBJC24400);the Young Scholars Program of Shandong University,Mizutani Foundation for Glycoscience,and Central Government Guide Local Science and Technology Development Funds(No.YDZX20203700002579).

摘　　要：A convenient and highly efficient strategy was developed for the simplification of enzymatic synthesis and purification of oligosaccharides.Glycosyl acceptors terminated with a thiol tag were extended by enzymatic modular assembly(EMA)in the aqueous phase,and the desired products were captured during solid-phase workup(SPW)using commercially available thiopropyl Sepharose 6B resin through a reversible disulfide linkage.Finally,the products were released from solid-phase resin in the presence of dithio-threitol(DTT)as reducing agent and the resin could be recovered and reused.This strategy overcomes the uncertain influence of polymer-or ionic-supports commonly used in enzymatic glycosylation,and showed perfect compatibility with all 10 enzyme modules for the rapid assembly of a series of complex oligosaccharides.

关 键 词：GLYCOSYLATION Oligosaccharide synthesis Thiol tag Capture-release BIOCATALYSIS 

分 类 号：O62[理学—有机化学]