胰腺癌差异表达基因的筛选及其预后价值:生物信息学分析  

Screening of differentially expressed genes and their prognostic value in pancreatic cancer:Bioinformatics analysis

作　　者：陈辉[1] 刘鹏[1] 王怀涛[1] 周磊[1] 谭晓冬[1] CHEN Hui;LIU Peng;WANG Huaitao;ZHOU Lei;TAN Xiaodong(Department of General Surgery,Shengjing Hospital of China Medical University,Liaoning Shenyang 110000,China)

机构地区：[1]中国医科大学附属盛京医院普通外科,辽宁沈阳110000

出　　处：《现代肿瘤医学》2022年第8期1425-1430,共6页Journal of Modern Oncology

基　　金：辽宁省自然科学基金资助项目(编号:20180530068)。

摘　　要：目的:基于生物信息学方法通过大样本挖掘胰腺导管腺癌(pancreatic ductal adenocarcinoma,PDAC)发生发展的关键基因。从公开生物数据库中挖掘PDAC的关键基因,探讨其在PDAC中的表达情况和预后价值,为PDAC的诊断和靶向治疗奠定理论基础。方法:从基因表达汇编(Gene Expression Omnibus,GEO)和癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库分别下载基因芯片数据GSE16515、GSE28735,GSE62452和TCGA-GTEx,通过R语言计算差异基因,寻找共同差异基因,并且GO富集分析和KEGG通路分析,构建蛋白质相互作用(protein-protein interaction,PPI)网络,筛选关键基因,最后验证关键基因在PDAC中的表达情况以及与PDAC预后的关系。结果:总共鉴定了219个DEGs,其中139个表达上调,80个表达下调。最终筛选出10个PDAC关键基因,其中FN1、POSTN、VCAN、MMP1、EGF和ALB与PDAC不良预后显著相关(P<0.05),进一步发现VCAN联合ALB、MMP1和POSTN具有更高预测PDAC生存预后的价值。结论:通过公开生物数据库挖掘出的与PDAC预后显著相关的5个关键基因,为PDAC发病机制、临床诊断和治疗靶点的研究提供理论依据。Objective:To provide theoretical basis for the diagnosis and targeted therapy of pancreatic ductal adenocarcinoma(PDAC),we excavated the hub genes of PDAC and investigated the expression and prognostic value via bioinformatics analysis.Methods:Gene chip data GSE16515,GSE28735,GSE62452 and TCGA-GTEx were downloaded from GEO and TCGA databases,respectively,then we found common differential expressed genes through R software.GO enrichment analysis and KEGG pathway analysis were conducted,and protein-protein interaction(PPI)Network was constructed to screen hub genes,and finally we verified the expression of hub genes in PDAC and their prognostic value of PDAC.Results:A total of 219 DEGs were identified,of which 139 were up-regulated and 80 were down-regulated.Finally,a total of 10 key genes associated PDAC were screened,among which FN1,POSTN,VCAN,MMP1,EGF,and ALB were significantly related to the poor prognosis of PDAC(P<0.05).VCAN combined with ALB,MMP1 and POSTN had a higher value in predicting the survival and prognosis of PDAC.Conclusion:The five hub genes are significantly related to the prognosis of PDAC unearthed from the public biological database,which provides a theoretical basis for future research on the pathogenesis,clinical diagnosis and treatment targets of PDAC.

关 键 词：胰腺导管腺癌 生物信息学 差异表达基因 关键基因 

分 类 号：R735.9[医药卫生—肿瘤]