杜仲叶清除DPPH自由基动力学特性及抗氧化活性成分筛选  

DPPH Kinetic Characteristics and Identification of Antioxidant Compounds in Eucommia ulmoides Leaves

作　　者：屠万倩[1] 张留记[1] 夏曼玉 李宁[2] 李向阳[3] TU Wan-qian;ZHANG Liu-ji;XIA Man-yu;LI Ning;LI Xiang-yang(Henan Engineering Center of Genuine Me-dicinal Materials Comprehensive Development,Henan Academy of Chinese Medicine,Zhengzhou450004,China;Henan University of Traditional Chinese Medicine,Zhengzhou450008,China;Henan Institute for Food and Drug Control,Zhengzhou450018,China)

机构地区：[1]河南省中医药研究院,河南省道地药材综合开发工程技术研究中心,郑州450004 [2]河南中医药大学,郑州450008 [3]河南省食品药品检验所,郑州450018

出　　处：《中国药学杂志》2022年第4期264-268,共5页Chinese Pharmaceutical Journal

基　　金：河南省科技厅工程技术研究中心建设项目资助(2020338);河南省中医药科学研究专项课题资助(2019ZYZD08)。

摘　　要：目的 研究杜仲叶抗氧化清除1,1-二苯基-2-苦肼基(DPPH)自由基的动力学特性,筛选其抗氧化活性成分,建立与抗氧化活性相关的质量控制方法。方法 比较不同杜仲叶样品浓度、反应温度和反应时间对DPPH自由基清除率的影响,测定杜仲叶清除DPPH自由基活性的半数清除率(IC;)。采用DPPH-HPLC法测定杜仲叶与DPPH反应前后的主要化学成分含量变化。结果 杜仲叶的样品浓度、反应温度和反应时间均对DPPH自由基清除率有一定影响,杜仲叶对DPPH自由基清除率随样品浓度的增加、反应温度的提高、反应时间的延长而增加。杜仲叶清除DPPH自由基的IC;为0.509 mg·mL^(-1)。与DPPH自由基反应后,杜仲叶提取液中桃叶珊瑚苷、京尼平苷酸含量降低较少,绿原酸、芦丁、异槲皮苷和紫云英苷的含量明显降低,提示绿原酸、芦丁、异槲皮苷和紫云英苷可能是杜仲叶清除DPPH自由基的主要抗氧化活性成分。结论 本研究初步明确杜仲叶清除DPPH自由基的动力学特性,筛选出了抗氧化活性成分,为建立与抗氧化活性相关的杜仲叶质量控制方法提供参考依据。OBJECTIVE To study the dynamic characteristics of DPPH scavenging activity of Eucommia ulmoides leaves, and identify the antioxidant active compounds. METHODS The influence of different concentrations of E. ulmoides leaves, reaction temperature and reaction time were compared. IC;of DPPH free radical scavenging rate was determined. DPPH-HPLC was used to analyze the content changes of the main compounds in E. ulmoides leaves before and after reaction with DPPH. RESULTS The concentrations of E. ulmoides leaves, reaction temperature and reaction time could affect DPPH free radical scavenging rate. The DPPH free radical scavenging rate of E. ulmoides leaves increased with the raise of concentration E. ulmoides leaves, reaction temperature and reaction time correspondingly. The IC;of DPPH free radical scavenging rate was 0.509 mg·mL^(-1). Compared with the contents before reaction with DPPH, the contents of aucubin, geniposidic acid in E. ulmoides leaves decreased little;while the contents of chlorogenic acid, rutin, isoquercitrin and astragalin decreased significantly. It suggested that chlorogenic acid, rutin, isoquercitrin and astragalin might be main antioxidant compounds in E. ulmoides leaves. CONCLUSION The dynamic characteristics of DPPH free radical scaveging activity of E. ulmoides leaves are preliminarily known. The antioxidant compounds in Eucommia ulmoides leaves were identified, which could provide reference for quality control of E. ulmoides leaves related to antioxidant activity.

关 键 词：杜仲叶 抗氧化成分 1 1-二苯基-2-苦肼基 动力学 

分 类 号：R282[医药卫生—中药学]