淡豆豉提取物不同溶剂萃取物的抗氧化活性  

Study on antioxidant activity of extracts from different polar solvents of extract of fermented soya beans

作　　者：刘俊泽 胡力铭 王仁广 王淑敏[1] 王欢 LIU Jun-ze;HU Li-ming;WANG Ren-guang;WANG Shu-min;WANG Huan(Changchun University of Chinese Medicine,Changchun,Jilin 130117,China)

机构地区：[1]长春中医药大学,吉林长春130117

出　　处：《食品与机械》2022年第3期184-188,196,共6页Food and Machinery

基　　金：长春中医药大学自然科学研究发展基金项目(编号:2019026)。

摘　　要：目的:探究淡豆豉粗提物的不同溶剂萃取物的成分差异、抗氧化活性。方法:以70%乙醇为提取溶剂,利用超声波法对淡豆豉粉末进行提取,挥干溶剂后得粗提物,用水分散,依次用石油醚、二氯甲烷、乙酸乙酯、正丁醇进行萃取,得到不同溶剂萃取物。测定粗提物与不同溶剂萃取物中总黄酮、总多糖含量;运用HPLC法测定大豆苷等6种大豆异黄酮含量,并分析其对DPPH自由基、ABTS自由基、OH自由基的清除作用。结果:乙酸乙酯萃取物中总黄酮含量最高,为(92.912±1.130)mg/g萃取物;正丁醇萃取物中总多糖含量最高,为(83.381±0.754)mg/g萃取物;正丁醇萃取物中异黄酮总含量高于粗提物及其他萃取物;淡豆豉粗提物及各溶剂萃取物均表现出体外抗氧化活性,其中正丁醇萃取物的体外抗氧化活性最强;总黄酮含量与5种大豆异黄酮含量和抗氧化活性呈显著相关性(P<0.05)。结论:正丁醇萃取物对3种自由基表现出较强的清除作用,可作为天然抗氧化剂加以利用。Objective:To explore the difference in composition and antioxidant activity of crude extracts of fermented soya beans with different solvent extracts.Methods:70%ethanol was used the extraction solvent,the fermented soya beans powder was extracted by ultrasonic method.After the solvent was evaporated,the crude extract was obtained.The crude extract was dispersed in water and extracted with petroleum ether,dichloromethane,ethyl acetate,and n-butanol in turn to obtain different solvent extracts.The colorimetry was used to determine the content of total flavonoids and total polysaccharides in crude extracts and different solvent extracts,HPLC was used to determine the content of 6 kinds of soybean isoflavones including daidzein.Then their scavenging effects were studied on DPPH free radicals,ABTS free radicals and OH free radicals,and the correlation was analyzed between antioxidant capacity and total flavonoids,total polysaccharides,and 6 kinds of soybean isoflavones.Results:The ethyl acetate extract had the highest total flavonoid content,which was(92.912±1.130)mg/g extract;The n-butanol extract had the highest total polysaccharide content,which was(83.381±0.754)mg/g extract,and the total content of isoflavones in the n-butanol extract was higher than the crude extract and other extracts.The crude extract of fermented soya beans and various solvent extracts showed in vitro antioxidant activity,among which n-butanol extract had the strongest in vitro antioxidant activity.The content of total flavonoids was significantly correlated with the content of 5 soybean isoflavones and antioxidant activity(P<0.05).Conclusion:The n-butanol extract had a strong scavenging effect on three kinds of free radicals and could be used as a natural antioxidant.

关 键 词：淡豆豉 萃取物 总黄酮 总多糖 异黄酮 抗氧化活性 

分 类 号：TS214[轻工技术与工程—粮食、油脂及植物蛋白工程] R284.2[轻工技术与工程—食品科学与工程]