热灭活乳酸菌TMC0356对代谢综合征大鼠肝脏脂代谢的影响及可能机制  

Effects of heat-inactivated Lactobacillus gasseri TMC0356 on liver lipid metabolism in rats with metabolic syndrome and its possible mechanism

作　　者：税启航 石磊[2] 何方[3] 李鸣[3] 黄承钰[3] 景小凡[2] 胡雯[1] SHUI Qihang;SHI Lei;HE Fang;LI Ming;HUANG Chengyu;JING Xiaofan;HU Wen(West China School of Medicine,Sichuan University,Chengdu,Sichuan 610041,P.R.China;Department of Clinical Nutrition,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,P.R.China;West China School of Public Health,Sichuan University,Chengdu,Sichuan 610041,P.R.China)

机构地区：[1]四川大学华西临床医学院,成都610041 [2]四川大学华西医院临床营养科,成都610041 [3]四川大学华西公共卫生学院,成都610041

出　　处：《华西医学》2022年第3期437-442,共6页West China Medical Journal

摘　　要：目的探讨热灭活乳酸菌TMC0356对代谢综合征(metabolic syndrome,MS)大鼠肝脏脂代谢的影响及其可能的作用机制。方法选择雄性Sprague-Dawley大鼠60只,随机分为基础组、MS模型组和高、中、低剂量热灭活乳酸菌TMC0356实验组。各组以不同的饮食连续喂养7 d,饲养15周后解剖取出肝脏,检测肝脏中过氧化物酶体增生物激活受体-α(peroxisome proliferator activated receptor-α,PPAR-α)、固醇调节元件结合蛋白-1c(sterol regulatory element binding protein-1c,SREBP-1c)、脂肪酸合成酶(fatty acid synthase,FAS)、肉碱脂酰转移酶-1(carnitine palmitoyltransferase-1,CPT-1)等基因的mRNA及蛋白表达量。结果5组间的PPAR-α、SREBP-1c、CPT-1的mRNA表达量比较,差异均无统计学意义(P>0.05)。低剂量TMC0356实验组FAS的mRNA表达量低于MS模型组(P=0.011)、中剂量TMC0356实验组(P=0.042)、高剂量TMC0356实验组(P=0.009);其余组间两两比较,差异均无统计学意义(P>0.05)。PPAR-α、SREBP-1c、FAS的蛋白表达量在5组间比较,差异均无统计学意义(P>0.05)。低剂量TMC0356实验组CPT-1的蛋白表达量高于基础组(P=0.033)、高剂量TMC0356实验组(P=0.043);其余组间两两比较差异均无统计学意义(P>0.05)。结论热灭活乳酸菌TMC0356可以改善MS大鼠代谢紊乱的症状,这可以通过多种方式发生,如抑制MS大鼠的食欲,增加胰岛素抵抗,下调FAS、SREBP-1c等。Objective To explore the effects of heat-inactivated Lactobacillus gasseri TMC0356 on liver lipid metabolism in rats with metabolic syndrome(MS)and its possible mechanism.Methods Sixty male Sprague-Dawley rats were selected.Rats were randomly divided into 5 groups,including control group,MS model group and three TMC0356 test groups(low-,medium-and high-dose groups).The rats in each group were fed with different diets for7 days,and the liver was dissected and removed after 15 weeks.The mRNA and protein expression levels of peroxisome hyperbioactive receptor-α(PPAR-α),sterol regulatory element binding protein-1c(REBP-1c),fatty acid synthase(FAS)and carnitine lipoacyltransferase-1(CPT-1)genes in liver were detected.Results There was no significant difference in the mRNA expression of PPAR-α,SREBP-1c or CPT-1 among the five groups(P>0.05).The mRNA expression of FAS in low-dose TMC0356 test group was lower than that in MS model group(P=0.011),medium-dose TMC0356 test group(P=0.042)and high-dose TMC0356 test group(P=0.009).There was no significant difference in the expression of FAS m RNA between other groups(P>0.05).There was no significant difference in the protein expression of PPAR-α,SREBP-1c or FAS among the five groups(P>0.05).The protein expression of CPT-1 in low-dose TMC0356 test group was higher than that in control group(P=0.033)and high-dose TMC0356 test group(P=0.043).There was no significant difference in the protein expression of CPT-1 between the other groups(P>0.05).Conclusion Heat-inactivated Lactobacillus gasseri TMC0356 may improve the symptoms of metabolic disorder in rats by suppressing appetite,improving insulin resistance,and downregulating the expression of key fat metabolism genes such as FAS and SREBP-1c.

关 键 词：代谢综合征 热灭活乳酸菌TMC0356 脂肪酸合成酶 固醇调节元件结合蛋白-1C 肉碱脂酰转移酶-1 过氧化物酶体增生物激活受体-α 

分 类 号：R589[医药卫生—内分泌]