玉米肽结合有氧运动抗肥胖作用及分子机制  

Anti-obesity Effect and Molecular Mechanism of Corn Peptide Combined with Aerobic Exercise

作　　者：张杉杉 尹凯波 姚晓雪 王碧辉 卢文丽 李美琪 杨金梅 孙婴宁[1] ZHANG Shanshan;YIN Kaibo;YAO Xiaoxue;WANG Bihui;LU Wenli;LI Meiqi;YANG Jinmei;SUN Yingning(College of Life Science and Agriculture Forestry,Qiqihar University,Qiqihar 161000,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161000

出　　处：《云南农业大学学报（自然科学版）》2022年第2期278-284,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(31402061);黑龙江省省属高等学校基本科研业务费科研项目(植物性食品加工技术特色学科专项)(YSTSXK201875);齐齐哈尔大学创新创业训练计划项目(202010232172,202010232166)。

摘　　要：【目的】研究玉米肽结合有氧运动对高脂饮食诱导小鼠肥胖的抑制作用及其分子机制。【方法】4周龄ICR小鼠随机分为标准对照组(CON,饲喂标准饲料)、高脂组(DIO)和高脂运动组(DEX)(均饲喂高脂饲料)、高脂玉米肽组(DCP)和高脂玉米运动肽组(DCE)(均饲喂添加玉米肽的高脂饲料),同时DEX组和DCE组每周进行6次有氧运动;喂养8周后,测量小鼠体质量、肾周脂肪、附睾脂肪、皮下脂肪以及肝脏组织质量,检测血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)和高密度脂蛋白(HDL)含量;Westernblot法检测脂肪细胞分化转录调控因子(PPARγ和C/EBPα)与脂质合成相关基因(FAS)的表达水平。【结果】单纯补充玉米肽对小鼠体质量、肝脏和脂肪组织质量、血脂水平和脂质合成相关基因表达具有一定的抑制作用,但不显著(P>0.05)。玉米肽结合有氧运动能降低或显著降低高脂饮食诱导的肥胖小鼠体质量、肝脏和脂肪组织质量(P>0.05或P<0.05),显著降低小鼠血清中TG和LDL含量(P<0.05),显著抑制PPARγ的表达水平(P<0.05)。【结论】玉米肽结合有氧运动能够抑制脂肪细胞分化转录调控因子PPARγ表达,对高脂饮食小鼠有抗肥胖作用。本研究为开发玉米肽作为运动减肥的辅助功能性食品提供了科学依据。[Purpose]To study the inhibitory effect of corn peptide combined with aerobic exercise on obesity mice induced by high-fat diet, and to explore the molecular mechanism of the effect.[Methods]Four-week-old ICR mice were randomly divided into standard control group(CON,feeding standard diet), high-fat group(DIO) and high-fat combined with aerobic exercise group(DEX)(both feeding high-fat diet), high-fat combined with corn peptide group(DCP) and the high-fat combined with corn peptide and aerobic exercise group(DCE)(both feeding the high-fat diet supplemented with corn peptide). At the same time, DEX and DCE group underwent aerobic exercise six times a week. After eight weeks of feeding, body weight, perirenal fat, epididymal fat, subcutaneous fat and liver tissue weight of mice were measured. Total cholesterol(TC), triglyceride(TG), low density lipoprotein(LDL) and high density lipoprotein(HDL) content in serum were detected. The expression levels of genes related with adipogenesis(PPARγ and C/EBPα) and lipogenesis(FAS) were determined by Western-blot. [Results]Dietary supplementation of corn peptide had a certain inhibitory effect on the body weight, liver and fatty tissue weight, lipid level and expression of lipid synthesis related genes in liver and adipose tissue of mice, but not significant(P>0.05). Corn peptide combined with aerobic exercise could reduce or significantly reduce the body weight, liver and fatty tissue weight(P>0.05 or P<0.05), significantly reduce serum TG and LDL content(P<0.05), and also significantly inhibited the expression level of PPARγ in obese mice(P<0.05). [Conclusion]Corn peptide combined with aerobic exercise can inhibit the expression of adipocyte differentiation transcriptional regulator PPARγ, which has anti-obesity effect on the high-fat diet mice. This study provides a scientific basis for the development of corn peptide as an auxiliary functional food for weight loss through exercise.

关 键 词：玉米肽 运动 高脂饮食 肥胖 

分 类 号：S513.099[农业科学—作物学]