蓝靛果花色苷的分离纯化及抗氧化能力  

Separation and purification and antioxidant activity of anthocyanin from Lonicera caerulea

作　　者：李梦莎[1] 周琳[1] 王化[1] 朱良玉[1] 何丹娆[1] 周丽萍[1] LI Mengsha;ZHOU Lin;WANG Hua;ZHU Liangyu;HE Danrao;ZHOU Liping(Institute of Nature and Ecology,Heilongjiang Academy of Sciences,Harbin 150040,Heilongjiang,China)

机构地区：[1]黑龙江省科学院自然与生态研究所,黑龙江哈尔滨150040

出　　处：《经济林研究》2022年第1期142-149,177,共9页Non-wood Forest Research

基　　金：国家“十三五”重点研发项目(2016YFC500304-07);黑龙江省科学院青年创新基金项目(CXJQ2019ZR02;CXJQ2020ZR02);黑龙江省自然科学基金联合引导项目(LH2019C078);黑龙江省院所基本应用技术研究专项(ZNBZ2020ZR05);黑龙江省科学院院基金国际合作重点项目(GH2021-ZR01)。

摘　　要：【目的】比较分析不同纯化方法对蓝靛果花色苷的提取得率及抗氧化能力的影响情况,以筛选出最佳的纯化工艺,为蓝靛果花色苷的深加工与高值化利用提供理论参考依据。【方法】以蓝靛果栽培品种‘蓝精灵’的成熟果实为研究对象,采用超声波辅助乙醇提取法制备其提取液后,采用色谱柱层析技术与静态吸附解析法,筛选能适用于其花色苷分离与纯化的固定相介质;再采用动态吸附和洗脱试验,对分离与纯化的工艺条件进行优化;然后分别测定纯化后各组分中花色苷的含量及其清除羟自由基和DPPH自由基能力与总抗氧化能力,并分析了花色苷的抗氧化能力与其纯度之间的相关性。【结果】1)筛选出的效果最优的大孔树脂为XDA-7型树脂,其达到吸附平衡和解析平衡的时间分别为30与90 min,其吸附率与解析率分别达到83.5%和85.3%;最适宜的洗脱体积为3 BV,最适用的洗脱液是体积分数为70%的乙醇水溶液;通过纯化得到花色苷的4个组分,根据洗脱顺序将其分别命名为L1、L2、L3、L4,将其粗提物命名为L0。2)花色苷各组分的纯度由大到小依次为L2>L3>L1>L4>L0;纯化后的蓝靛果花色苷各组分的总抗氧化能力由大到小依次为L2>L3>L1>L4,其对羟自由基的清除能力由强至弱依次为L1>L4>L3>L0,其对DPPH自由基的清除能力由强至弱依次为L4>L2>L3>L1>L0。3)花色苷的纯度与其总抗氧化能力之间呈显著正相关;而与其清除羟自由基、DPPH自由基的IC50值之间均呈负相关,但其相关性均不显著(P<0.05)。【结论】采用优化得到的纯化工艺提取花色苷,具有较高的提取率,且蓝靛果花色苷的纯度与其抗氧化能力之间呈现出正相关关系。优化得到的纯化工艺是一种可用于其深加工的较为理想的纯化工艺。【Objective】By comparing the effects of different purification methods on the extraction yield and antioxidant capacity of anthocyanins in Lonicera edulis,and screen the optimal purification technology in order to provide theoretical reference for the deep processing and high value utilization of anthocyanins in L.edulis.【Method】Taking the mature fruit of L.edulis cultivar as the research object,the extract was prepared by ultrasonic-assisted ethanol extraction method,and then the suitable separation and purification stationary phase medium was selected by column chromatography technology and static adsorption analysis;the recapture dynamic adsorption and elution test optimizes the separation and purification conditions.The anthocyanin content,hydroxyl free radical scavenging ability,DPPH free radical scavenging ability and total reducing ability in the purified components were determined respectively and analyzed the correlation between the antioxidant capacity of anthocyanins and their purity.【Result】1)The most effective macroporous resin was XDA-7,which reached adsorption equilibrium at 30 minutes;reached analytical equilibrium at 90 minutes;and its adsorption rate and resolution rate are 83.5%and 85.3%,respectively;the best elution volume was 3 BV,and the best elution liquid integral was 70%ethanol aqueous solution.Optimized through purification conditions,four anthocyanin components were obtained,which were named as L1,L2,L3 and L4 according to the elution order,and the crude extract was named as L0.2)In terms of the antioxidant effect of purified L.edulis anthocyanins,the total antioxidant capacity of each component was L2>L3>L1>L4>L0;the total antioxidant capacity of the purified anthocyanins from L.indigo was L2>L3>L1>L4,and the scavenging ability to hydroxyl radicals was L1>L4>L3>L0,as well as its scavenging ability to DPPH free radicals was L4>L2>L3>L1>L0.3)There was a significant positive correlation between the purity of anthocyanins and total antioxidant capacity.There was negative correla

关 键 词：蓝靛果 花色苷 大孔树脂 纯化 体外抗氧化 

分 类 号：S663[农业科学—果树学] S609.2[农业科学—园艺学]