葛仙米藻胆蛋白和藻蓝蛋白对S180荷瘤小鼠肿瘤生长的影响及其作用机制  

Effects of Phycobiliprotein and Phycocyanin from Nostoc sphaeroides Kützing on the Growth of S180 Tumor-bearing Mice and Its Mechanism

作　　者：余佳 陈颖[2] 马淑梅 张瑞华[1] 王玉兰 YU Jia;CHEN Ying;MA Shumei;ZHANG Ruihua;WANG Yulan(Hunan Micro Algae Biological Engineering Technology Research Center,Hunan Yandi Bioengineering Co.,Ltd.,Zhuzhou 412007,China;Hunan University of Technology,Zhuzhou 412007,China;China State Institute of Pharmaceutical Industry,Shanghai 200040,China;State Key Laboratory of New Drug&Pharmaceutial Process,Shanghai Institute of Pharmaceutial Industry,Shanghai 200437,China)

机构地区：[1]湖南炎帝生物工程有限公司,湖南省微藻生物工程技术研究中心,湖南株洲412007 [2]湖南工业大学,湖南株洲412007 [3]中国医药工业研究总院,上海200040 [4]上海医药工业研究院创新药物与制药工艺国家重点实验室,上海200437

出　　处：《食品工业科技》2022年第8期399-405,共7页Science and Technology of Food Industry

基　　金：湖南省自然科学基金(2020JJ6096);湖南省教育厅科学研究项目(19C0550)。

摘　　要：目的:对人工养殖的葛仙米藻胆蛋白和藻蓝蛋白在S180荷瘤小鼠肿瘤生长的影响及作用机制研究。方法:通过考察S180荷瘤小鼠肿瘤体积、小鼠脏器指数和肿瘤组织切片观察,对葛仙米中藻胆蛋白、藻蓝蛋白的体内抗肿瘤活性进行评价,并初步研究了其作用机制。结果:体内抗肿瘤作用结果显示,葛仙米藻胆蛋白12.5、25、50 mg/kg剂量组对小鼠肉瘤S180的抑瘤率分别为11.19%、20.14%、37.65%。葛仙米藻蓝蛋白12.5、25、50 mg/kg剂量组对小鼠肉瘤S180的抑瘤率分别为19.74%、35.00%、52.06%。藻蓝蛋白能够明显促进抑癌蛋白P53和促凋亡蛋白Bax的表达,抑制凋亡抑制蛋白Bcl2的表达。结论:葛仙米藻蓝蛋白高剂量组可显著减少S180肉瘤细胞的增殖,葛仙米藻蓝蛋白低、中剂量和葛仙米藻胆蛋白高剂量也有一定的抑制作用,人工养殖的葛仙米中藻胆蛋白和藻蓝蛋白具有较好的体内抗肿瘤活性。Objective:Effects of phycobiliprotein and phycocyanin from artifically raised Nostoc sphaeroides Kützing on the growth of tumor in S180 tumor-bearing mice and its mechanism.Methods:The in vivo antitumor activity of phycobiliprotein and phycocyanin from Nostoc sphaeroides Kützing was evaluated by examining the tumor volume of S180 tumor-bearing mice,mouse organ indices and tumor tissue section observation,and its mechanism of action was initially studied.Results:The results of in vivo antitumor effect showed that the tumor inhibition rates of phycobiliprotein 12.5,25and 50 mg/kg dose groups were 11.19%,20.14% and 37.65%,respectively,against mouse sarcoma S180.The tumor inhibition rates of phycocyanin 12.5,25 and 50 mg/kg dose groups were 19.74%,35.00% and 52.06%,respectively,against mouse sarcoma S180.Phycocyanin significantly promoted the expression of oncoprotein P53 and pro-apoptotic protein Bax,and inhibited the expression of apoptosis suppressor protein Bcl2.Conclusion:Phycocyanin high dose group significantly reduced the proliferation of S180 sarcoma cells.Phycocyanin low and medium doses and phycocyanin high doses also had some inhibitory effects.Phycobiliprotein and phycocyanin from artifically raised Nostoc sphaeroides Kützing have better in vivo antitumor activity.

关 键 词：葛仙米 藻胆蛋白 藻蓝蛋白 S180荷瘤小鼠 抗肿瘤 

分 类 号：Q955[生物学—动物学]