两种银耳多糖对小鼠抗衰作用的比较  

Comparison of Anti-Aging Effects Between Two Tremella fuciformis Polysaccharides

作　　者：徐晓飞 罗东辉 赵明月 钟兴伟 XU Xiaofei;LUO Donghui;ZHAO Mingyue;ZHONG Xingwei(College of Food Science and Engineering,Guangdong Ocean University,Yangjiang 529599,Guangdong,China;South China Institute of Collaborative Innovation,Dongguan 523808,Guangdong,China)

机构地区：[1]广东海洋大学食品科学与工程学院,广东阳江529599 [2]华南协同创新研究院,广东东莞523808

出　　处：《食用菌学报》2022年第2期93-102,共10页Acta Edulis Fungi

基　　金：广东省重点领域研发计划(2021B0707060001)。

摘　　要：为研究银耳(Tremella fuciformis)多糖(polysaccharides,TFP)对D-半乳糖致衰小鼠抗衰作用,分析两种商业化银耳多糖WSK和BETA的总糖含量、糖醛酸含量、空间构象和重均分子量(weight-average molecular weight,M_(w))分布等性质,利用腹腔注射D-半乳糖(120 mg·kg^(-1))构建衰老小鼠模型,比较100 mg·kg^(-1)的WSK和BETA对小鼠皮肤中羟脯氨酸和透明质酸含量、组织中抗氧化酶活性和脂质过氧化产物含量、血清中炎症因子水平、肠道pH和短链脂肪酸(short chain fatty acids,SCFAs)含量的影响。结果表明:WSK和BETA的总糖(干重)含量分别为91.2%和89.5%;糖醛酸(干重)含量分别为20.2%和19.3%;M_(w)分别为10.83×10^(6)和3.59×10^(6);WSK和BETA均不含三螺旋结构。与WSK相比,BETA可极显著提高小鼠皮肤中的羟脯氨酸和透明质酸含量、小鼠肝脏和心脏中的SOD和GSH-Px活性(P<0.01);极显著降低小鼠肝脏和心脏中的MDA含量、血清中的IL-1β和TNF-α含量(P<0.01);显著提高小鼠血清中的SOD活性(P<0.05)。WSK和BETA均可部分逆转D-半乳糖所致结肠pH和总SCFAs含量的改变。与WSK相比,BETA对D-半乳糖致衰小鼠抗皮肤老化、降低组织氧化压力和抗炎作用效果更好。Two commercially available Tremella fuciformis polysaccharides(TFP),WSK and BETA,were determined for total sugar content,uronic acid content,structural characteristics,weight-average molecular weight(M_(w)),and then studied for their anti-aging effect at a dose of 100 mg·kg^(-1) in aging mice established by intraperitoneal injection of 120 mg·kg^(-1) D-galactose.WSK and BETA were obtained by hot water-enzyme extraction and hot water extraction,respectively.Each treatment group was measured for contents of hydroxyproline and hyaluronic acid in mouse skin,antioxidant enzyme activity and MDA concentration in mouse serum,liver and heart,levels of inflammatory factors in mouse serum,intestinal pH,and concentrations of intestinal short chain fatty acids(SCFAs).The results showed that the total sugar contents(dry weight)of WSK and BETA were 91.2%and 89.5%,respectively.The uronic acid contents of WSK and BETA were 20.2%and 19.3%,respectively.The M w of WSK and BETA were 10.83×10^(6) and 3.59×10^(6),respectively.Neither of the two polysaccharides contained triple helix structure.Compared with WSK,BETA increased hydroxyproline and hyaluronic acid contents(P<0.01),SOD activity in serum(P<0.05)liver and heart(P<0.01),and GSH-Px activity in liver and heart(P<0.01).In contrast,BETA decreased MDA levels in liver and heart(P<0.01),and IL-1βand TNF-αlevels in serum(P<0.01)compared with WSK.Both WSK and BETA partially reversed the changes of pH in colon and total SCFAs concentration due to aging.The results suggested that BETA showed better anti-aging effects on skin aging,tissue oxidative stress and inflammation than WSK.

关 键 词：银耳多糖 D-半乳糖致衰小鼠 抗氧化 抗衰老 重均分子量 

分 类 号：TS201.4[轻工技术与工程—食品科学]