Valproic acid enhances neurosphere formation in cultured rat embryonic cortical cells through TGFβ1 signaling  

作　　者：Cui Qi Jiaqi Zhang Yuanyuan Wang Mingyan Lin Jun Gao Haiying Lu 

机构地区：[1]Department of Neurobiology,Key Laboratory of Human Functional Genomics of Jiangsu,School of Basic Medical Sciences,Nanjing Medical University,Nanjing,Jiangsu 211166,China [2]Department of Rehabilitation Medicine,Jiangsu Shengze Hospital affiliated to Nanjing Medical University,Suzhou,Jiangsu 215228,China [3]Department of Neurology,Children's Hospital of Nanjing Medical University,Nanjing,Jiangsu 210008,China

出　　处：《The Journal of Biomedical Research》2022年第2期127-140,共14页生物医学研究杂志（英文版）

基　　金：This work was supported by grants from the Key R&D Program of Jiangsu Province(Grant No.2017CX010);the National Natural Science Foundation of China(Grant No.81973308)to J.G.;the Nanjing Science and Technology Development Program(Grant No.201402021)to H.L.

摘　　要：This study aimed to investigate the effect and mechanism of valproic acid(VPA)on the neurosphere formation in rat embryonic cortical cells.We used free-floating neurosphere formation as a model system to evaluate the VPA on the proliferation of neural stem cells(NSCs).We found a time-and dose-dependent increase in neurosphere formation and NSC proliferation after VPA treatment.Further RNA-seq analysis demonstrated that the upregulated TGFβ1 signaling might attribute to the effect of VPA on the neurosphere formation and NSC proliferation.Consistently,the neurosphere formation and NSC proliferation were blocked by the treatment with SB431542,an inhibitor of TGFβ1 receptor.Moreover,in a coculture system,NSCs treated with VPA significantly reduced the oxygen-glucose deprivation-induced neuronal apoptosis.Taken together,our results showed that VPA could enhance neurosphere formation and NSC proliferation by activating TGFβ1,which might be a novel therapeutic strategy for neurological disorders.

关 键 词：valproic acid neural stem cells neurosphere formation PROLIFERATION transforming growth factorβ1 

分 类 号：R741[医药卫生—神经病学与精神病学]