pH对红色红曲菌(Monascus ruber)M7生长及产孢的影响  

Effect of p H on the growth and sporulation of Monascus ruber M7

作　　者：董钰鑫 陈莎[1] 高梦祥[1] 李利[1] DONG Yuxin;CHEN Sha;GAO Mengxiang;LI Li(College of Life Science,Yangtze University,Jingzhou 434025,China)

机构地区：[1]长江大学生命科学学院,湖北荆州434025

出　　处：《中国酿造》2022年第3期76-80,共5页China Brewing

基　　金：国家自然科学基金资助项目(31730068)。

摘　　要：研究红色红曲菌(Monascus ruber)M7生长、繁殖两个不同阶段对环境pH需求的差异,在孢子培养过程中对pH进行分段控制,旨在减小产孢条件对菌丝生长的影响,提高孢子产量。结果表明,菌株M7孢子在pH 3.0~6.0条件下培养8 h均可100%萌发,其中,pH4.0~5.0最佳,萌发最为迅速,在pH 7.0~8.0时孢子萌发受到严重抑制,萌发率很低;菌落生长在pH 4.0~6.0时较快,其中,pH 5.0最佳,菌落直径最大,但在pH 3.0和7.0时菌落生长明显减慢,在pH 8.0时受到严重抑制,形成的菌落很小;pH>8.0的碱性条件为极端pH条件,孢子萌发和菌落生长完全被抑制。在菌株M7生长的pH范围内,产孢能力随着pH的升高而提高,适宜的产孢pH范围为7.0~8.0。根据菌株M7生长和繁殖对pH的需求差异,控制培养前期pH值为5.0、后期pH值为7.0~8.0,在PDB和CYB培养基中的产孢量分别提高了12~16倍和3~4倍。The difference of environmental pH requirements of Monascus ruber M7 at two different stages of growth and reproduction was studied,and pH was subsection controlled during spore culture, in order to reduce the influence of sporulation conditions on mycelium growth and increase spore yield. The results showed that the spores of strain M7 could germinate 100% when cultured for 8 h under pH 3.0-6.0, among them, pH 4.0-5.0was the optimal and the germination was the fastest, spore germination was severely inhibited and the germination rate was very low under pH7.0-8.0. Colony growth was faster at pH 4.0-6.0, among them, pH 5.0 was the optimal, and the colony diameter was the largest. However, the colony growth slowed down significantly at pH 3.0 and 7.0, and severely inhibited at pH 8.0, forming a small colony. The alkaline condition with pH>8.0were extreme pH conditions, and spore germination and colony growth were completely inhibited. In the pH range of strain M7 growth, the sporulation capacity increased with the increase of pH, and the suitable sporulation pH range was 7.0-8.0. Based on the differences of pH requirement for growth and reproduction of strain M7, the spore production in PDB and CYB medium increased by 12-16 times and 3-4 times respectively by controlling the pH in the early stage of culture to be 5.0 and the pH in the later stage to be 7.0-8.0.

关 键 词：红色红曲菌 PH 孢子萌发 菌落生长 产孢培养 

分 类 号：TQ925.1[轻工技术与工程—发酵工程]