基于斑马鱼模型的茶多酚及儿茶素单体降糖作用研究  

Study on the Hypoglycemic Effect of Tea Polyphenols and Catechins Based on Zebrafish Model

作　　者：谭蓉[1,2] 刘均 李强[1,2] TAN Rong;LIU Jun;LI Qiang(Hangzhou Tea Research Institute,CHINA COOP,Hangzhou 310016,China;Zhejiang Key Laboratory of Transboundary Applied Technology for Tea Resources,Hangzhou 310016,China)

机构地区：[1]中华全国供销合作总社杭州茶叶研究院,浙江杭州310016 [2]浙江省茶资源跨界应用技术重点实验室,浙江杭州310016

出　　处：《中国茶叶加工》2022年第1期71-78,共8页China Tea Processing

摘　　要：斑马鱼是一种新型模式生物,已成为生命科学研究的新宠。研究以茶多酚及其儿茶素单体为对象,采用胰岛素依赖型糖尿病(IDDM)斑马鱼模型开展了茶多酚及儿茶素单体的降糖效应比较研究。结果发现,15~45μg/mL茶多酚、EGCG、EGC、EC和ECG(以生药量计)均能够降低斑马鱼葡萄糖值水平,但其降糖效应弱于糖尿病治疗药物阿卡波糖。相同生药量下(30μg/mL),降糖作用的强弱依次是:ECG>EGCG>EC>茶多酚>EGC,酯型儿茶素降糖效应稍强于非酯型。通过斑马鱼在体实验研究,表明茶多酚及主要儿茶素均具有明显的降糖效应。Zebrafish is a new model organism and has received increasing attention in life science research.In this study,the hypoglycemic effect of tea polyphenols and four catechin monomers were evaluated based on the insulin dependent diabetes mellitus(IDDM)zebrafish.The results showed that 15~45μg/mL tea polyphenols,EGCG,EGC,EC and ECG(measured by raw drug weight to liquid volume ratio)could reduce the glucose level of zebrafish,but its hypoglycemic effect was weaker than that of the diabetes drug acarbose.Under the same drug dosage(30μg/mL),the order of hypoglycemic effect was:ECG>EGCG>EC>tea polyphenols>EGC,and the hypoglycemic effect of ester catechins was slightly stronger than that of non-ester catechins.The in vivo study of zebrafish showed that both tea polyphenols and major catechins had obvious hypoglycemic effects.

关 键 词：斑马鱼 糖尿病 降糖作用 儿茶素 

分 类 号：Q946.84[生物学—植物学]