Minocycline Activates the Nucleus of the Solitary Tract- Associated Network to Alleviate Lipopolysaccharide-Induced Neuroinflammation  

作　　者：Jianbo Xiu Lanlan Li Qi Xu 

机构地区：[1]State Key Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences,School of Basic Medicine Peking Union Medical College,Beijing 100005,China [2]Neuroscience Center,Chinese Academy of Medical Sciences,Beijing 100005,China

出　　处：《Chinese Medical Sciences Journal》2022年第1期1-14,共14页中国医学科学杂志（英文版）

基　　金：supported by the research grants from the National Natural Science Foundation of China(81625008 and 31970952);the National Key Research and Development Program of China(2020YFA0804500,2020YFA0804502)。

摘　　要：Objective To examine the neuroanatomical substrates underlying the effects of minocycline in alleviating lipopolysaccharide(LPS)-induced neuroinflammation.Methods Forty C57BL/6 male mice were randomly and equally divided into eight groups.Over three conse-cutive days,saline was administered to four groups of mice and minocycline to the other four groups.Immediately after the administration of saline or minocycline on the third day,two groups of mice were additionally injected with saline and the other two groups were injected with LPS.Six or 24 hours after the last injection,mice were sacrificed and the brains were removed.Immunohistochemical staining across the whole brain was performed to detect microglia activation via Iba1 and neuronal activation via c-Fos.Morphology of microglia and the number of c-Fo-positive neurons were analyzed by Image-Pro Premier 3D.One-way ANOVA and Fisher’s least-significant differences were employed for statistical analyses.Results Minocycline alleviated LPS-induced neuroinflammation as evidenced by reduced activation of microglia in multiple brain regions,including the shell part of the nucleus accumbens(Acbs),paraventricular nucleus(PVN)of the hypothalamus,central nucleus of the amygdala(CeA),locus coeruleus(LC),and nucleus tractus solitarius(NTS).Minocycline significantly increased the number of c-Fo-positive neurons in NTS and area postrema(AP)after LPS treatment.Furthermore,in NTS-associated brain areas,including LC,lateral parabrachial nucleus(LPB),periaqueductal gray(PAG),dorsal raphe nucleus(DR),amygdala,PVN,and bed nucleus of the stria terminali(BNST),minocycline also significantly increased the number of c-Fo-positive neurons after LPS administration.Conclusion Minocycline alleviates LPS-induced neuroinflammation in multiple brain regions,possibly due to increased activation of neurons in the NTS-associated network.

关 键 词：NEUROINFLAMMATION LIPOPOLYSACCHARIDE depression nucleus tractus solitaries MICROGLIA 

分 类 号：R965[医药卫生—药理学]