灭活副干酪乳杆菌TK1501发酵红枣饮品对葡聚糖硫酸钠诱导的慢性结肠炎改善作用  

Improved effect of inactivated Lactobacillus paracasei TK1501 fermented red jujube drink on chronic colitis induced by DSS

作　　者：刘成琪 霍姝伊 王宁宇 胡梅梅 牛新月 王海宽[1] LIU Chengqi;HUO Shuyi;WANG Ningyu;HU Meimei;NIU Xinyue;WANG Haikuan(College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《食品与发酵工业》2022年第7期58-63,共6页Food and Fermentation Industries

基　　金：宁夏回族自治区重点研发计划项目(2018BBF02008)。

摘　　要：旨在研究副干酪乳杆菌TK1501发酵的灭活型红枣饮品对葡聚糖硫酸钠(dextran sulfate sodium,DSS)诱导的慢性结肠炎(ulcerative colitis,UC)模型小鼠的改善作用。将40只无特定病原体(specific pathogen free,SPF)级健康雄性BALB/c小鼠,随机分为4组,分别为空白组、模型组、实验组和对照实验组。通过分析UC小鼠的结肠长度、疾病活动指数(disease activity index,DAI)评分、促炎因子含量、抗炎因子含量、紧密连接蛋白表达量和结肠病理改变,探究了副干酪乳杆菌TK1501发酵的灭活型红枣饮品对DSS诱导的结肠炎小鼠的保护效果。结果显示,TK1501发酵的红枣饮品能够显著(P<0.05)降低结肠炎小鼠的DAI评分,缓解结肠萎缩症状,改善病理结构。此外,发酵饮品还能够上调结肠炎小鼠细胞中抗炎因子IL-4的含量,下调促炎因子IL-1β、IL-6含量,显著增加紧密连接蛋白Occludin、黏蛋白MUC-2的表达水平。副干酪乳杆菌TK1501发酵的红枣饮品对慢性结肠炎具有一定的改善作用,是一款具有开发价值的功能性饮品。To study the improvement effect of inactivated jujube beverage fermented by Lactobacillus paracasei TK1501 on dextran sulfate sodium(DSS)-induced ulcerative colitis mice.Forty male BALB/c mice of SPF were randomly divided into four groups:blank group,model group,experimental group and control group.By testing the length,the disease activity index(DAI),pro-inflammatory cytokines,anti-inflammatory cytokines,the expression of tight junction proteins and the pathological changes in colon.The results showed that the fermented jujube beverage significantly(P<0.05)reduced the DAI score and improve the symptoms of colon atrophy and the pathological structure in colitis mice.In addition,the fermented beverage up-regulate the anti-inflammatory cytokines IL-4 and down-regulate the pro-inflammatory cytokines IL-1βand IL-6 in colitis mice,and increase the expression levels of tight junction protein Occludin and mucin MUC-2 significantly.Thus,jujube beverage fermented by L.paracasei TK1501 has a certain improvement effect on ulcerative colitis,which is a functional beverage with development value and significance.

关 键 词：慢性结肠炎 副干酪乳杆菌 红枣 后生素 灭活菌 

分 类 号：TS275.4[轻工技术与工程—农产品加工及贮藏工程]