基于网络药理学探讨固肾安胎方治疗先兆性流产的作用机制  

Mechanism study of treating threatened abortion with Gushen Antai formula based onnetwork pharmacology

作　　者：柯仲成[1,2] 王秦武 王玉强 邓太友 陈敏楠 陈向阳[1] KE Zhong-cheng;WANG Qin-wu;WANG Yu-qiang;DENG Tai-you;CHEN Min-nan;CHEN Xiangyang(Research Center of Chinese medicine efficacy and health technology,Huangshan University,Anhui Huangshan 245041,China;College of Pharmacy,Nanjing University of Chinese Medicine,Jiangsu Nanjing 210028,China;Beijing Anting Pharmaceutical Biotechnology Co.,Ltd.,Beijing 101500,China;Huangshan Mandinew Pharmaceutical Co.,Ltd.,Anhui Huangshan 245700,China)

机构地区：[1]黄山学院中药功效与健康技术中心,安徽黄山245041 [2]南京中医药大学药学院,江苏南京210028 [3]北京安汀医药生物科技有限公司,北京101500 [4]黄山曼迪新药业有限公司,安徽黄山245700

出　　处：《中国医院药学杂志》2022年第6期617-621,共5页Chinese Journal of Hospital Pharmacy

基　　金：安徽省自然科学基金面上项目(编号:2008085MH269);黄山学院校企合作科技重大专项(编号:hxkt2021036);安徽教育厅省级教学研究团队(编号:2020jxtd250)。

摘　　要：目的:通过网络药理学方法探讨固肾安胎方治疗先兆性流产的作用机制。方法:运用TCMSP、SwissTarget等数据库筛选化学成分、获取潜在靶点,构建药材-成分-靶点网络图,并进行信号通路的富集与分析,利用分子对接技术探究潜在靶点与活性成分的相互作用。结果:获得固肾安胎方的核心成分89个和作用靶点34个,涉及Steroidogenesis、PI3K-Akt等23条信号通路;分子对接结果表明槲皮素、山姜素、山柰酚与CYP1B1、ACHE、PGF等靶蛋白具有良好的结合能力。结论:网络药理学揭示了固肾安胎方可通过多个靶点、多个信号通路来治疗先兆性流产,为进一步研究其作用机制提供了理论基础。OBJECTIVE To explore the molecular mechanism of Gushen Antai formula in treatment of threatened abortion by network pharmacology.METHODS The active constituents and target proteins were obtained from TCMSP,SwissTarget databases,medicinal material-component-target network map was constructed,and the pathway enrichment was performed for molecular mechanism analysis,then,molecular docking was used to explore the interaction between potential targets and active components.RESULTS 89 active components with 34 potential targets were screened out,which were involved in 23 signal pathways,such as steroidogenesis,PI3K-Akt signal pathway.The docking results showed that main active ingredients of Gushen Antai formula had good binding abilities with key target proteins.CONCLUSION Through the network pharmacology,active components and the mechanism of Gushen Antai formula in the treatment of threatened abortion were revealed,which provided a theoretical basis for further research on its mechanism of action.

关 键 词：网络药理学 固肾安胎方 先兆性流产 分子对接 

分 类 号：R285.5[医药卫生—中药学]