新鞘氨醇单胞菌预处理对木材结构及酶解的影响  

Effect of Novosphingobium sp.Pretreatment on the Structure and Enzymolysis of Two Kinds of Wood

作　　者：曹舒祺 陈梦婕 侯贤锋 孙瑾 CAO Shu-qi;CHEN Meng-jie;HOU Xian-feng;SUN Jin(College of Material and Energy,South China Agricultural University,Guangzhou 510642,Guangdong,P.R.China)

机构地区：[1]华南农业大学材料与能源学院,广东广州510642

出　　处：《林产工业》2022年第4期9-13,共5页China Forest Products Industry

基　　金：2021年广州市农业和社会发展科技计划“废木质资源化处理研究与示范”项目(202103000011);国家重点研发计划“高韧性增速固化胶黏剂研发”(2018YFD0600305);广州市科技计划项目“利用城市废弃木质家具制造功能性再生人造板技术攻关”(201803030031)。

摘　　要：采用新鞘氨醇单胞菌(Novosphingobium sp.)分别对针叶材(马尾松)和阔叶材(尾叶桉)进行处理。通过化学成分分析、傅里叶变换红外光谱(FT-IR)分析、X射线衍射(XRD)分析、扫描电子显微镜(SEM)观察、酶解糖化测试,研究细菌预处理对针叶材和阔叶材的化学结构、微观形态、酶解效率等的影响。结果表明:新鞘氨醇单胞菌预处理破坏了木材中的木质素结构,致使结晶指数增大,增加了纤维素的可及性,提高了木材的酶解效果。相对于针叶材,新鞘氨醇单胞菌预处理对阔叶材的木质素及微观结构破坏更为明显。In this study,Novosphingobium sp.was used to treat softwood(Pinus massoniana)and hardwood(Eucalyptus urophylla),respectively.Through composition test analysis,Fourier transform infrared spectroscopy(FT-IR),X-ray diffractometer analysis(XRD),scanning electron microscope(SEM),enzymatic saccharification test,the effects of bacterial pretreatment on the chemical structrue,microscopic morphology,and enzymolysis efficiency of softwood and hardwood were evaluated.The results showed that Novosphingobium sp.pretreatment destroyed the lignin structure in wood,resulting in an increase in the crystallization index and the accessibility of cellulose,and improved the enzymatic hydrolysis effect of the wood.Compared with softwood,the pretreatment of Novosphingobium sp.damaged the lignin and microstructure of hardwood more obviously.

关 键 词：细菌预处理 新鞘氨醇单胞菌 尾叶桉 马尾松 酶解 

分 类 号：TS6[轻工技术与工程] S718[农业科学—林学]