In Vitro Inhibition of Alphaviruses by Lycorine  

作　　者：Na Li Zhen Wang Rui Wang Zhe-Rui Zhang Ya-Nan Zhang Cheng-Lin Deng Bo Zhang Lu-Qing Shang Han-Qing Ye 

机构地区：[1]Key Laboratory of Special Pathogens and Biosafety,Center for Biosafety Mega-Science,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China [2]College of Pharmacy and Drug Discovery Center for Infectious Diseases,Nankai University,Tianjin 300350,China

出　　处：《Virologica Sinica》2021年第6期1465-1474,共10页中国病毒学（英文版）

基　　金：This work was supported by the National Key Research and Development Program of China(2018YFA0507201)。

摘　　要：Chikungunya virus(CHIKV) is a mosquito-borne alphavirus. As an emerging virus, CHIKV imposes a threat to public health. Currently, there are no vaccines or antivirals available for the prevention of CHIKV infection. Lycorine, an alkaloid from Amaryllidaceae plants, has antiviral activity against a number of viruses such as coronavirus, flavivirus and enterovirus. In this study, we found that lycorine could inhibit CHIKV in cell culture at a concentration of 10 lmol/L without apparent cytotoxicity. In addition, it exhibited broad-spectrum anti-alphavirus activity, including Sindbis virus(SINV),Semliki Forest virus(SFV), and Venezuelan equine encephalomyelitis virus(VEEV). The time of addition studies indicated that lycorine functions at an early post-entry stage of CHIKV life cycle. The results based on two different CHIKV replicons provided further evidence that lycorine exerts its antiviral activity mainly by inhibiting CHIKV translation.Overall, our study extends the antiviral spectrum of lycorine.

关 键 词：Chikungunya virus(CHIKV) ALPHAVIRUS LYCORINE ANTIVIRAL 

分 类 号：R285[医药卫生—中药学]