Engineered neutrophil apoptotic bodies ameliorate myocardial infarction by promoting macrophage efferocytosis and inflammation resolution  

作　　者：Lili Bao Geng Dou Ran Tian Yajie Lv Feng Ding Siying Liu Ruifeng Zhao Lu Zhao Jun Zhou Lin Weng Yan Dong Bei Li Shiyu Liu Xin Chen Yan Jin 

机构地区：[1]State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi International Joint Research Center for Oral Diseases,Center for Tissue Engineering,School of Stomatology,The Fourth Military Medical University,Xi’an,710032,China [2]School of Chemical Engineering and Technology,Shaanxi Key Laboratory of Energy Chemical Process Intensification,Institute of Polymer Science in Chemical Engineering,Xi’an Jiao Tong University,Xi’an,710049,China [3]Department of Dermatology,Tangdu Hospital,The Fourth Military Medical University,Xi’an,710038,China [4]State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi International Joint Research Center for Oral Diseases,Center for Digital Dentistry,School of Stomatology,The Fourth Military Medical University,Xi’an,Shaanxi,710032,China [5]State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Clinical Research Center for Oral Diseases,Department of Oral and Maxillofacial Surgery,School of Stomatology,The Fourth Military Medical University,Xi’an,Shaanxi,710032,China

出　　处：《Bioactive Materials》2022年第3期183-197,共15页生物活性材料（英文）

基　　金：This work was supported by National Key Research and Development Program of China(2016YFC1101400);National Natural Science Foundation of China(31800817);Innovative Talent Project of Shaanxi province(2020KJXX-057);National Natural Science Foundation of China(31870970);National Natural Science Foundation of China(81991504);Key Research and Development Program of Shaanxi Province(2019SF-073);Young Talent Support Program of Stomatology of FMMU(41741835-MZ2020D5).

摘　　要：Inflammatory response plays a critical role in myocardial infarction(MI)repair.The neutrophil apoptosis and subsequent macrophage ingestion can result in inflammation resolution and initiate regeneration,while the therapeutic strategy that simulates and enhances this natural process has not been established.Here,we constructed engineered neutrophil apoptotic bodies(eNABs)to simulate natural neutrophil apoptosis,which regulated inflammation response and enhanced MI repair.The eNABs were fabricated by combining natural neutrophil apoptotic body membrane which has excellent inflammation-tropism and immunoregulatory properties,and mesoporous silica nanoparticles loaded with hexyl 5-aminolevulinate hydrochloride(HAL).The eNABs actively targeted to macrophages and the encapsulated HAL simultaneously initiated the biosynthesis pathway of heme to produce anti-inflammatory bilirubin after intracellular release,thereby further enhancing the anti-inflammation effects.In in vivo studies,the eNABs efficiently modulated inflammation responses in the infarcted region to ameliorate cardiac function.This study demonstrates an effective biomimetic construction strategy to regulate macrophage functions for MI repair.

关 键 词：NEUTROPHILS Apoptotic bodies Engineering INFLAMMATION Myocardial infarction 

分 类 号：R54[医药卫生—心血管疾病]