基于微生物学的速食食品致病菌检验研究  

Research on pathogenic bacteria detection of fast food based on microbiology

作　　者：王辉[1] 张亮[1] 周美静[1] WANG Hui;ZHANG Liang;ZHOU Meijing(Baoding Disease Prevention and Control Center,Baoding 071000,Hebei,China)

机构地区：[1]保定市疾病预防控制中心,河北保定071000

出　　处：《粘接》2022年第4期84-87,共4页Adhesion

摘　　要：分析基于微生物学的速食食品致病菌检验检测结果,总结相应的检验方法。采用传统细菌分离培养法和实时荧光定量PCR法进行检验。对两种检验方法下的致病菌阳性检出情况进行统计,计算出总阳性率。并对两组检验所耗费的时间进行记录,计算出平均用时结果。经统计与组间比较,实时荧光定量PCR法的样本阳性检出率为11.00%,较传统细菌分离培养法的8.00%明显较高,P<0.05。对比不同检验方法下的用时情况,可得实时荧光定量PCR法的平均用时为(2.35±0.12)h,明显短于传统细菌分离培养法的(37.35±0.15)h,P<0.05。基于微生物学的速食食品致病菌检验检过程中应用不同的检验方法均能获得一定的检验结果,其中实时荧光定量PCR法的检验效果更为理想。To analyze the test results of pathogenic bacteria in fast-food food based on microbiology and summarize the corresponding test methods.Traditional bacterial isolation and culture method and real-time fluorescent quantitative PCR method were used for detection.Count the positive detection of pathogenic bacteria under the two test methods,and calculate the total positive rate.Meanwhile,record the time spent in the two sets of tests,and calculate the average time.According to statistics and comparison between groups,the positive detection rate of real-time fluorescent quantitative PCR method was 11.00%,which was significantly higher than the 8.00%of traditional bacterial isolation and culture method,and P<0.05.Comparing the time under different test methods,the average time of real-time fluorescent quantitative PCR method is(2.35±0.12)h,which is significantly shorter than the(37.35±0.15)h of traditional bacterial isolation and culture method,and P<0.05.In the process of microbiology-based fast food pathogenic bacteria detection,the application of different inspection methods can obtain certain inspection results,and the detection effect of real-time fluorescent quantitative PCR method is more ideal.

关 键 词：速食食品 致病菌 检验 实时荧光定量PCR法 传统细菌分离培养法 

分 类 号：TS207[轻工技术与工程—食品科学]