醉金香葡萄愈伤细胞悬浮培养基优化促进白藜芦醇的合成  

Optimization of Callus Suspension Culture Medium for Enhancing Resveratrol Biosynthesis in Vitis vinifera Grape

作　　者：王晓惠 王泽建[1] 肖慈英[1] 刘泽波 郭美锦[1] 庄英萍[1] WANG Xiaohui;WANG Zejian;XIAO Ciying;LIU Zebo;GUO Meijin;ZHUANG Yingping(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《华东理工大学学报（自然科学版）》2022年第2期203-212,共10页Journal of East China University of Science and Technology

基　　金：国家重点研发专项(2020YFA0906800)。

摘　　要：建立和优化了一种醉金香葡萄悬浮细胞合成白藜芦醇的工艺。以细胞生长和白藜芦醇生物合成量为响应值,通过单因素实验、Plackett-Burman、响应面实验考察了不同碳源、氮源、前体、诱导剂和激素等因素对醉金香葡萄细胞悬浮培养基合成白藜芦醇的影响。结果表明,在醉金香葡萄悬浮细胞的最佳培养条件下,单位葡萄细胞中白藜芦醇的最高表达量为(3026.64±56)μg/g,与对照组相比提高了51.13倍,为后续实现利用醉金香葡萄细胞悬浮培养生产白藜芦醇提供了理论基础。Resveratrol,a secondary metabolite of stilbene,is synthesized by the L-Phenylalanine pathway in plants.Under external conditions,various plants are used to produce resveratrol.However,the most commonly used plant cell cultures for the production of stilbene are still from grapes.In this paper,an optimal technology for the production of resveratrol from suspension cell of Vitis vinifera Loes.was established.In response to cell growth and resveratrol biosynthesis,the effects of different carbon sources,nitrogen sources,precursors,inducers and hormones on resveratrol synthesis in the grape cells were studied by single factor test,Plackett-Burman test and response surface methodology.The results showed that the optimal nutrient conditions of suspension cells were as follows:B5 medium 5 g/L,sucrose 37.5 g/L,yeast extract 900 mg/L,phenylalanine 100 mg/L,methyl jasmonate 84μmol/L,2,4-chlorophenoxyacetic acid 4.1 mg/L,6-benzylaminopurine 0.2 mg/L,polyvinylpyrrolidone 1.0 g/L.Under these conditions,the highest expression of resveratrol was(3026.64±56)μg/g in cell suspension culture at 26°C for 7 d,which was 51.13 times higher than that before optimization,the optimal nutrient conditions can significantly promote the synthesis of resveratrol.In this study,the nutrient composition of suspension cells of Vitis vinifera was studied in details,and the optimal culture conditions for the growth and resveratrol synthesis of the suspension cells were determined.On the one hand,it provides a basis for analyzing the function of grape suspension cells,and on the other hand,it lays a good foundation for scale-up the production of resveratrol and other substances.

关 键 词：醉金香葡萄 愈伤细胞 悬浮培养 营养组分优化 白藜芦醇 

分 类 号：TS201.3[轻工技术与工程—食品科学]