Anti-scarring effect of sodium hyaluronate at filtration pathway after filtering surgery in rabbits  

作　　者：Yu-Jue Wang Le-Meng Feng Cheng Zhang Wu-Long Zhang Wei-Tao Song 

机构地区：[1]National Clinical Research Center for Geriatric Diseases,Xiangya Hospital of Central South University,Changsha 410008,Hunan Province,China [2]Eye Center of Xiangya Hospital,Central South University,Changsha 410008,Hunan Province,China [3]Hunan Key Laboratory of Ophthalmology,Changsha 410008,Hunan Province,China

出　　处：《International Journal of Ophthalmology(English edition)》2022年第4期547-553,共7页国际眼科杂志（英文版）

基　　金：Supported by National Natural Science Fundation of China(No.81974132;No.81770927);National Key R&D Program of China(No.2021YFA1101202);Hunan Provincial Health Commission(No.202207022839)。

摘　　要：AIM: To investigate the anti-scarring effect of sodium hyaluronate(HA) at filtration pathway after filtering surgery in a rabbit model.METHODS: Fifteen healthy adult New Zealand white rabbits were selected for trabeculectomy in both eyes. The right eyes were used as HA group with 0.1 m L HA injected into the anterior chamber at the end of the operation;the left eyes were used with 0.1 m L sodium lactate Ringer’s solution(RS) injected into the anterior chamber as RS group. Intraocular pressure(IOP), filtering blebs morphology, inflammatory reaction and complications were observed at the 7, 60, and 90 d after surgery.RESULTS: One day after surgery, the IOP of HA and RS groups were 12.75±1.92 and 10.50±1.59 mm Hg(P=0.005). At the 7;day postoperative, the filtering blebs of each group were functional type and TGF-β expression was significantly difference in both groups(0.10±0.01 vs 0.14±0.02, P=0.024). After 60 d of the operation, all filtering blebs were scarring and alpha-smooth muscle actin(α-SMA) expression was significantly difference in both groups(0.40±0.04 vs 0.35±0.02, P=0.032). α-SMA positive cells were mainly distributed in the junction of conjunctiva and sclera and around the blood vessels. The collagen volume fraction(CVF) of HA and RS group was(75.49±7.01)% and(79.93±5.35)%(P=0.044). On the 90;day after the operation, CVF was(82.57±5.19)% and(88.08±1.75)% in HA and RS groups(P=0.036). There was no α-SMA positive cell in HA group, while a few positive cells were observed in RS group(P=0.000).CONCLUSION: HA has effect of anti-scar and antiinflammation on filtration pathway after filtering surgery within 3 mo by inhibiting fibroblast proliferation and collagen deposition.

关 键 词：sodium hyaluronate filtering surgery antiscarring GLAUCOMA 

分 类 号：R779.6[医药卫生—眼科]