3D-printed dermis-specific extracellular matrix mitigates scar contraction via inducing early angiogenesis and macrophage M2 polarization  

作　　者：Lei Chen Zhiyong Li Yongtai Zheng Fei Zhou Jingling Zhao Qiyi Zhai Zhaoqiang Zhang Tianrun Liu Yongming Chen Shaohai Qi 

机构地区：[1]Department of Burns,Laboratory of General Surgery,The First Affiliated Hospital,SunYat-Sen University,Guangzhou,510080,China [2]School of Materials Science and Engineering,Centre of Functional Biomaterials,Key Laboratory of Polymeric Composite Materials and Functional Materials of Ministry of Education,GD Research Centre for Functional Biomaterials Engineering and Technology,Sun Yat-sen University,Guangzhou,510275,China [3]Department of Oral and Maxillofacial Surgery,Stomatological Hospital,Southern Medical University,No.366,South of Jiangnan Boulevard,Guangzhou,510280,China [4]Department of Otorhinolaryngology Head and Neck Surgery,The Sixth Affiliated Hospital of Sun Yat-sen University,Guangzhou,Guangdong,China

出　　处：《Bioactive Materials》2022年第4期236-246,共11页生物活性材料（英文）

摘　　要：Scar contraction frequently happens in patients with deep burn injuries.Hitherto,porcine dermal extracellular matrix(dECM)has supplied microenvironments that assist in wound healing but fail to inhibit scar contraction.To overcome this drawback,we integrate dECM into three-dimensional(3D)-printed dermal analogues(PDA)to prevent scar contraction.We have developed thermally gelled,non-rheologically modified dECM powder(dECMp)inks and successfully transformed them into PDA that was endowed with a micron-scale spatial structure.The optimal crosslinked PDA exhibited desired structure,good mechanical properties as well as excellent biocompatibility.Moreover,in vivo experiments demonstrated that PDA could significantly reduced scar contraction and improved cosmetic upshots of split thickness skin grafts(STSG)than the commercially available dermal templates and STSG along.The PDA has also induced an early,intense neovascularization,and evoked a type-2-like immune response.PDA’s superior beneficial effects may attribute to their desired porous structure,the well-balanced physicochemical properties,and the preserved dermis-specific ECM cues,which collectively modulated the expression of genes such as Wnt11,ATF3,and IL1β,and influenced the crucial endogenous signalling pathways.The findings of this study suggest that PDA is a clinical translatable material that possess high potential in reducing scar contraction.

关 键 词：Extracellular matrix Dermal analogues Scar contraction MACROPHAGE ANGIOGENESIS 

分 类 号：R318[医药卫生—生物医学工程] R62[医药卫生—基础医学]