提取方式对十字花科蔬菜中异硫氰酸酯的影响  

Effect of Different Extraction Methods on Isothiocyanate Contents Measured in Seeds of Cruciferous Vegetables

作　　者：张尧 Nyasha Makaza 吕城枝 孙娟 宋新杰 Sabir Z Nishanbaev 吴元锋[1,2] 毛建卫 ZHANG Yao;Nyasha Makaza;Lü Cheng-zhi;SUN Juan;SONG Xin-jie;Sabir Z Nishanbaev;WU Yuan-feng;MAO Jian-wei(School of Biological and Chemical Engineering,Zhejiang University of Science and Technology,Hangzhou 310023,Zhejiang,China;Zhejiang Provincial Collaborative Innovation Center for Biochemical Manufacturing of Agricultural Biological Resources(2011 Collaborative Innovation Center),Hangzhou 310023,Zhejiang,China;Institute of the Chemistry of Plant Substances,Academy of Sciences of the Republic of Uzbekistan,Tashkent 100170,Uzbekistan)

机构地区：[1]浙江科技学院生物与化学工程学院,浙江杭州310023 [2]浙江省农业生物资源生化制造协同创新中心(2011协同创新中心),浙江杭州310023 [3]乌兹别克斯坦科学院植物化学研究所,乌兹别克斯坦塔什干100170

出　　处：《食品研究与开发》2022年第8期47-53,共7页Food Research and Development

基　　金：浙江省重点研发计划(2019C02064);浙江省自然科学基金(LY16C200005)。

摘　　要：通过环缩合法和气质联用法探究先酶解后提取(hydrolysis followed by extraction,HFE)和酶解-提取同时进行(simultaneous hydrolysis and extraction,SHE)两种提取方式对6种十字花科蔬菜(萝卜、芥菜、榨菜、甘蓝、花菜和西兰花)种子中异硫氰酸酯(isothiocyanate,ITC)种类和含量的影响,并分析B16细胞抑制活性和NAD(P)H:醌氧化还原酶1[NAD(P)H:quinone oxidoreductase 1,NQO1]诱导活性受提取物影响的差异。环缩合法的结果表明,与HFE相比SHE可显著提高西兰花和萝卜种子ITC含量(P<0.05)。气相色谱-质谱联用的结果表明,SHE比HFE显著提高了萝卜种子中莱菔素(3.26倍)、erucin、raphasatin、4-MI和B,4-I-1,西兰花种子中erucin(1.63倍)和芥菜、甘蓝、花菜和西兰花种子中iberverin的含量(P<0.05)。与HFE相比,SHE增加了芥菜和西兰花种子中ITC的种类,分别为iberverin和octane(OCT),并减少了花菜种子中ITC的种类,cyclopropyl isothiocyanate(CP)。两种方法对alyssin、iberin等其他ITC种类和含量无显著影响(P>0.05)。细胞活性试验表明,SHE组萝卜、甘蓝、花菜和西兰花种子提取液的细胞抑制率均显著高于HFE组(P<0.05),并且SHE组西兰花种子的NQO1诱导活性显著高于HFE组(P<0.05)。Seeds from six kinds of cruciferous vegetables—turnip,leaf mustard,tuber mustard,cabbage,cauliflower,and broccoli—were used to study the effects of hydrolysis followed by extraction(HFE)and simultaneous hydrolysis and extraction(SHE)on isothiocyanate(ITC)types and their contents measured through cyclization and gas chromatography-mass spectrometry(GC-MS). The effects of extracts obtained using different extraction methods on B16 cell activity and NAD(P)H:quinone oxidoreductase 1(NQO1)activity in seeds were also analyzed. SHE resulted in significantly higher ITC contents measured in broccoli and turnip seeds than HFE(P<0.05). GC-MS measurements showed that SHE resulted in significantly higher contents of sulforaphane(3.26-fold),erucin,raphasatin,4-MI,and B,4-I-1 in turnip seeds;of erucin(1.63-fold)in broccoli seeds;and of iberverin in leaf mustard,cabbage,cauliflower,and broccoli seeds than HFE(P<0.05). SHE also resulted in higher contents of iberverin and octane measured in mustard and broccoli seeds,respectively,and lower contents of cyclopropyl isothiocyanate measured in cauliflower seeds than HFE. However,SHE had no significant effect on the contents of other ITC types such as alyssin and iberin(P >0.05). Furthermore,the rates of inhibition of B16 cell activity by turnip,cabbage,cauliflower,and broccoli seed extracts in the SHE group were significantly higher than those in the HFE group(P<0.05). The NQO1 activity of broccoli seeds in the SHE group was also significantly higher than that in the HFE group(P<0.05).

关 键 词：提取方式 十字花科蔬菜 异硫氰酸酯(ITC) 气相色谱-质谱 抗氧化活性 

分 类 号：TS255.3[轻工技术与工程—农产品加工及贮藏工程]