仙人掌果花色苷纯化工艺的研究  

Purification process of anthocyanin from opuntia ficus-indica

作　　者：张筠 邵帅[2] 吉雪慧 张瑞英 张守文[2] ZHANG Yun;SHAO Shuai;JI Xuehui;ZHANG Ruiying;ZHANG Shouwen(Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme,Harbin 150086;College of Food Engineering,Heilongjiang East University,Harbin 150066)

机构地区：[1]黑龙江省农业科学院博士后科研工作站,哈尔滨150086 [2]黑龙江东方学院食品工程学院,哈尔滨150066

出　　处：《中国食品添加剂》2022年第4期103-110,共8页China Food Additives

基　　金：黑龙江东方学院重点项目,HDFKY200105。

摘　　要：仙人掌果中含花色苷、黄酮等多种具有生物活性的物质,其中花色苷具有抗氧化、预防慢性疾病、抑制炎症,改善视力、调节肠道菌群等作用。为了对仙人掌果中花色苷进行深入研究,本文主要对其纯化工艺进行优化,选用HPD-100树脂,静态吸附与解析条件为:静态吸附时间为6h,上样液浓度为90mg/L,上样液pH2,静态解析时间为4h,解析液浓度为80%;动态吸附与解析条件为:柱床体积90mL,动态吸附流速为2mL/min,动态解析流速为1mL/min,除去可溶性杂质用水量为100mL。纯化后花色苷的色价是纯化之前9.33倍(P<0.05),纯化后花色苷的纯度是纯化之前的5.44倍(P<0.05)。通过高效液相色谱进行定量分析,得到仙人掌果粉中花色苷含量为806.4mg/100g(以矢车菊素-3-O-葡萄糖苷计)。Opuntia ficus-indica contains anthocyanins,flavonoids and other substances rich in biological activity.Among them,anthocyanins have the functions of anti-oxidation,preventing chronic diseases,inhibiting inflammation,improving eyesight,and regulating intestinal flora.In order to conduct an in-depth study on the anthocyanins,the crude anthocyanin solution was purified in this experiment,and HPD-100 resin was selected for purification.The static adsorption and analysis conditions were as follows:static adsorption time was 6h,and the concentration of the sample solution was 90mg/L,the pH of the sample solution was 2,the static analysis time was 4h,and the concentration of the analysis solution was 80%.The dynamic adsorption and analysis conditions were as follows:the column bed volume was 90mL,the dynamic adsorption flow rate was 2mL/min,and the dynamic analysis flow rate was 1mL/min,the amount of water used to remove soluble impurities was 100mL.After purification,the color value of anthocyanins increased significantly(P<0.05),which was 9.33 times before purification,and the purity of anthocyanins after purification was significantly improved(P<0.05),which was 5.44 times before purification.And the content of anthocyanin in opuntia ficus-indica powder was 806.4mg/100g(calculated as Cyanidin-3-glucoside)by high-performance liquid chromatography quantitative analysis.

关 键 词：仙人掌果 花色苷 纯化工艺 

分 类 号：TS202.3[轻工技术与工程—食品科学]