Involvement of NLRP3-inflammasome pathway in noise-induced hearing loss  

作　　者：Na Sai Yuan-Yuan Yang Long Ma Da Liu Qing-Qing Jiang Wei-Wei Guo Wei-Ju Han 

机构地区：[1]College of Otolaryngology Head and Neck Surgery,Chinese PLA General Hospital,Beijing,China [2]National Clinical Research Center for Otolaryngologic Diseases,Beijing,China [3]Key Laboratory of Hearing Science,Ministry of Education,Beijing,China [4]Beijing Key Laboratory of Hearing Impairment for Prevention and Treatment,Beijing,China [5]Department of Otolaryngology Head and Neck Surgery,Peking University Shenzhen Hospital,Shenzhen,Guangdong Province,China [6]PLA Rocket Force Characteristic Medical Center Department of Stomatology,China

出　　处：《Neural Regeneration Research》2022年第12期2750-2754,共5页中国神经再生研究（英文版）

基　　金：supported by the National Key Research and Development Program of China, No.2020YFC2005200(to WWG and WJH);the National Nature Science Foundation of China, Nos.81770992(to NS and WJH), 81970897(to WWG);Health and Family Planning System Research Project of Shenzhen Municipality, No.SZXJ2018079(to YYY);Shenzhen Sanming Project, No.SZSM201612076(to YYY)

摘　　要：The inflammasome is a multiprotein oligomer in the cell cytoplasm and is part of the innate immune system.It plays a crucial role in the pathological process of noise-induced hearing loss(NIHL).However,the mechanisms of NLR family pyrin domain containing 3(NLRP3)inflammasome activation in NIHL have not been clearly demonstrated.In this study,miniature pigs were exposed to white noise at 120 dB(A)and auditory brainstem response measurements were used to measure their hearing function.Immunofluorescence staining,confocal laser scanning microscopy,western blot assay,and quantitative reverse transcription-polymerase chain reaction were used to analyze inflammasome-related protein distribution and expression.NLRP3,interleukin-1β,interleukin-18,and cleaved-caspase-1 were highly expressed in the cochlea after 120 dB(A)white noise exposure.Our findings suggest that NLRP3-inflammasomes in the cochlea may be activated after acoustic trauma,which may be an important mechanism of noise-induced hearing loss.

关 键 词：acoustic injury INFLAMMASOME INTERLEUKIN-1Β miniature pig NLR family pyrin domain containing 3(NLRP3) noise-induced hearing loss sensorineural hearing loss 

分 类 号：R764[医药卫生—耳鼻咽喉科]