Acetyl-11-keto-beta-boswellic acid promotes sciatic nerve repair after injury: molecular mechanism  

作　　者：Yao Wang Zong-Liang Xiong Xiang-Lin Ma Chong Zhou Mo-Han Huo Xiao-Wen Jiang Wen-Hui Yu 

机构地区：[1]Department of Veterinary Medicine,Northeast Agricultural University,Harbin,Heilongjiang Province,China [2]College of Life Sciences,Northeast Agricultural University,Harbin,Heilongjiang Province,China [3]Key Laboratory of Heilongjiang Education Department for Common Animal Disease Prevention and Treatment,Northeast Agricultural University,Harbin,Heilongjiang Province,China [4]Institute of Chinese Veterinary Medicine,Northeast Agricultural University,Harbin,Heilongjiang Province,China

出　　处：《Neural Regeneration Research》2022年第12期2778-2784,共7页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China, No.31972725(to WHY)

摘　　要：Previous studies showed that acetyl-11-keto-beta-boswellic acid(AKBA),the active ingredient in the natural Chinese medicine Boswellia,can stimulate sciatic nerve injury repair via promoting Schwann cell proliferation.However,the underlying molecular mechanism remains poorly understood.In this study,we performed genomic sequencing in a rat model of sciatic nerve crush injury after gastric AKBA administration for 30 days.We found that the phagosome pathway was related to AKBA treatment,and brain-derived neurotrophic factor expression in the neurotrophic factor signaling pathway was also highly up-regulated.We further investigated gene and protein expression changes in the phagosome pathway and neurotrophic factor signaling pathway.Myeloperoxidase expression in the phagosome pathway was markedly decreased,and brain-derived neurotrophic factor,nerve growth factor,and nerve growth factor receptor expression levels in the neurotrophic factor signaling pathway were greatly increased.Additionally,expression levels of the inflammatory factors CD68,interleukin-1β,pro-interleukin-1β,and tumor necrosis factor-αwere also decreased.Myelin basic protein-andβ3-tubulin-positive expression as well as the axon diameter-to-total nerve diameter ratio in the injured sciatic nerve were also increased.These findings suggest that,at the molecular level,AKBA can increase neurotrophic factor expression through inhibiting myeloperoxidase expression and reducing inflammatory reactions,which could promote myelin sheath and axon regeneration in the injured sciatic nerve.

关 键 词：AKBA AXON genomics inflammatory injury and repair myelin sheath MYELOPEROXIDASE neurotrophic factor peripheral nerve phagosome pathway regeneration Sprague-Dawley rat 

分 类 号：R285.5[医药卫生—中药学]