Sphingolipids Profiling of Plasma in Patients with Diabetes Mellitus Associated with Atherosclerosis by a Novel Normal-Phase UPLC-QToF MS Method  

作　　者：Min Li Li Yang Yining Huang Yu Bai Huwei Liu 

机构地区：[1]Beijing Institute for Drug Control(Beijing Center for Health Food and Cosmetics Control),Beijing 102206,China [2]Beijing National Laboratory for Molecular Sciences,Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Institute of Analytical Chemistry,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China [3]Department of Neurology,Peking University First Hospital,Beijing 100034,China

出　　处：《Journal of Analysis and Testing》2017年第3期245-254,共10页分析检测（英文）

基　　金：the National Key R&D Program of China(2017YFC0906800);National Natural Science Foundation of China(Grant nos.21527809 and 21175005).

摘　　要：A novel normal-phase UPLC-QToF MS method was developed for sphingolipids analysis,which was then applied to separate and identify seven classes of sphingolipids in plasma.The time-consuming of this novel method was much shorter than previous 2D LC–MS method,while the sensitivity,repeatability and resolution for sphingolipids analysis were better or comparable.Besides,the differences of lipids in human plasma among diabetes mellitus patients,diabetes mellitus associated with atherosclerosis patients and control subjects were also compared.Finally,12 sphingolipids species were identified as the amounts of which in diabetes mellitus associated with atherosclerosis patients were significantly higher than them in diabetes mellitus patients(VIP>1.0,p value<0.05,fold change>1.5).As the isomers of GalCs and GluCs were successfully separated,it was found that five GluCs,rather than GalCs,were included in these 12 potential biomarkers.These results suggest the need to separate GalCs from GluCs,and also indicated that there may be some extra activation pathways of diabetes mellitus associated with atherosclerosis,different from the atherosclerosis pathway and the diabetes mellitus pathway,causing cardiovascular disease.

关 键 词：UPLC-QToF MS Diabetes mellitus ATHEROSCLEROSIS SPHINGOLIPIDS Lipidomics Cardiovascular disease 

分 类 号：R58[医药卫生—内分泌]