拟除虫菊酯类农药及其代谢物免疫检测技术研究进展  

Progress on the development of immunoassays for pyrethroids insecticides and their metabolites

作　　者：许梓红 罗林[1] 陈子键[1] 王炳志 严义勇 孙远明[1] 徐振林[1] XU Zihong;LUO Lin;CHEN Zijian;WANG Bingzhi;YAN Yiyong;SUN Yuanming;XU Zhenlin(Guangdong Provincial Key Laboratory of Food Quality and Safety,College of Food Science,South China Agricultural University,Guangzhou 510642,China;Bioeasy Technology,Inc.,Shenzhen 518000,China)

机构地区：[1]广东省食品质量安全重点实验室,华南农业大学食品学院,广东广州510642 [2]深圳市易瑞生物技术股份有限公司,广东深圳518000

出　　处：《食品与发酵工业》2022年第8期320-328,共9页Food and Fermentation Industries

基　　金：广东省普通高校重点研究项目(2019KJDXM002);广东省科技计划项目(2018A050506072)。

摘　　要：拟除虫菊酯类农药是一类在世界范围内被广泛用于防治害虫的神经毒剂。由于用量大且存在一定毒性,因此,研究适合现场快速检测的筛查方法对于保障消费者身心健康具有重要作用。基于抗体特异性识别的免疫分析法具有简便、灵敏、快速及低成本等特点,是当前食品安全主流快速检测方法。该文重点介绍了拟除虫菊酯类农药及其代谢物半抗原设计策略、抗体制备和免疫检测新方法等方面研究进展,并对其应用现状、存在的问题及未来前景进行了评述。Pyrethroid pesticides are a class of nerve agents that are widely used to control pests in the world.Due to the extensive usage and toxicity,it is essential to develop screening methods suitable for rapid detection in the field of the protection of physical and mental health of consumers.Immunoassay based on the specific interaction of antibody and antigen is characterized as simplicity,sensitivity,rapidity and low cost.It has been established as the main rapid method for food safety at present.This review focused on the research progress of immunoassay for pyrethroid pesticides and their metabolites,included the hapten design strategies,antibody preparation,as well as the development of novel immunoassay methods.The application status,existing problems and prospects were also briefly discussed.

关 键 词：拟除虫菊酯类 免疫法检测 半抗原设计 抗体制备 

分 类 号：TQ450.1[化学工程—农药化工]