洋紫荆花营养成分及其急性毒性分析  

Analysis of Nutritional Components and Acute Toxicity of Bau-hinia variegata

作　　者：王亚凤[1] 梁蒙 谢桃结 阳丙媛 彭京 何瑞杰[1] 朱鸿杰 黄永林[1] WANG Yafeng;LIANG Meng;XIE Taojie;YANG Bingyuan;PENG Jing;HE Ruijie;ZHU Hongjie;HUANG Yonglin(Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization,Guangxi Institute of Botany,Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin,Guangxi,541006,China;Liuzhou Institute of Gardening Science,Liuzhou,Guangxi,545005,China)

机构地区：[1]广西壮族自治区中国科学院广西植物研究所,广西植物功能物质与资源持续利用重点实验室,广西桂林541006 [2]柳州市园林科学研究所,广西柳州545005

出　　处：《广西科学院学报》2022年第1期69-75,共7页Journal of Guangxi Academy of Sciences

基　　金：柳州市科技攻关项目(2019B0801b006);广西科技重大专项项目(桂科AA18118015);广东省重点领域研发计划项目(2020B1111110003);中央引导地方科技发展专项(桂科ZY20111010)资助。

摘　　要：为评价洋紫荆(Bauhinia variegata)花的营养价值和急性毒性作用,本研究按照食品安全国家标准法对3种花色洋紫荆花的营养成分进行检测分析,并采用改良寇氏法测定洋紫荆花乙醇浸膏对小鼠的急性毒性作用,以期为洋紫荆花资源的开发利用提供理论依据。结果表明:在所测定的17种氨基酸中,3种花色洋紫荆花的氨基酸种类一致,同种氨基酸在3种花色洋紫荆花中含量差别不大;粉花洋紫荆中Fe的含量极显著低于红花和白花洋紫荆,Mn的含量显著高于红花和白花洋紫荆;粉花洋紫荆中维生素C的含量极显著高于红花和白花洋紫荆。急性毒性试验结果显示:红花洋紫荆乙醇浸膏的半数致死量(Lethal Dose 50%,LD_(50))为30.34 g/kg,95%平均可信限为27.97-32.91 g/kg;粉花洋紫荆乙醇浸膏的LD_(50)值为33.79 g/kg,95%平均可信限为30.43-37.53 g/kg;白花洋紫荆乙醇浸膏的LD_(50)值为30.24 g/kg,95%平均可信限为27.11-33.73 g/kg。根据WHO外源化合物经口急性毒性分级标准,3种花色洋紫荆花乙醇浸膏灌胃小鼠一次经口LD_(50)值均大于15 g/kg,毒性分级为无毒,为洋紫荆花可食用性的开发利用提供科学依据。In order to evaluate the nutritional value and acute toxicity of Bauhinia variegata,the nutritional components of three kinds of flower colors of B.variegata were tested and analyzed according to the National Food Safety Standard method,and the acute toxicity of ethanol extract of B.variegata flowers on mice was determined by modified karber's method.It hopes to provide theoretical basis for the development and utiliza-tion of B.variegata resources.The results showed that among the 17 amino acids determined,the amino acids of three kinds of flower colors of B.variegata were identical,and the content of the same amino acid in three kinds of flower colors of B.variegata had little difference.The content of Fe in pink flowers of B.var-iegata was significantly lower than that in safflowers of B.variegata and white flowers of B.variegata,and the content of Mn was significantly higher than that in safflowers of B.variegata and white flowers of B.variegata.The content of vitamin C in pink flowers of B.variegata was significantly higher than that in saf-flowers of B.variegata and white flowers of B.variegata.The results of the acute toxicity test showed that the LD_(50) value of safflowers of B.variegata ethanol extract was 30.34 g/kg and the 95%average confidence limit was 27.97-32.91 g/kg.The LD_(50) value of pink flowers of B.variegata ethanol extract was 33.79 g/kg and the 95%average confidence limit was 30.43-37.53 g/kg.The LD_(50) value of white flowers of B.varie-gata ethanol extract was 30.24 g/kg and the 95%average confidence limit was 27.11-33.73 g/kg.Accord-ing to the WHO oral acute toxicity grading standard of exogenous compounds,the LD_(50) value of three kinds of B.variegata ethanol extracts in mice were all greater than 15 g/kg in one oral administration,and the tox-icity was classified as non-toxic.The above results provide a scientific basis for the development and utiliza-tion of edible flower of B.variegata.

关 键 词：洋紫荆 营养成分 氨基酸 急性毒性 可食用性 

分 类 号：O629[理学—有机化学]