Exposure to trace levels of metals and fluoroquinolones increases inflammation and tumorigenesis risk of zebrafish embryos  

作　　者：Dantong Jia Ruijie Zhang Jian Shao Wei Zhang Leilei Cai Weiling Sun 

机构地区：[1]College of Environmental Sciences and Engineering,Peking University,The Key Laboratory of Water and Sediment Sciences,Ministry of Education,Beijing,100871,China [2]State Environmental Protection Key Laboratory of All Material Fluxes in River Ecosystems,International Joint Laboratory for Regional Pollution Control,Ministry of Education,Beijing,100871,China [3]College of Animal Science,Guizhou University,The Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Education,Guiyang,Guizhou,550025,China [4]Department of Plant,Soil and Microbial Sciences,Environmental Science and Policy Program,Michigan State University,East Lansing,MI,48824,United States [5]College of Safety and Environmental Engineering,Shandong University of Science and Technology,Qingdao,Shandong,266590,China

出　　处：《Environmental Science and Ecotechnology》2022年第2期12-22,共11页环境科学与生态技术（英文）

基　　金：the National Natural Science Foundation of China(Grant No.51925901 and 51879001).

摘　　要：Exposure to trace-level heavy metals and antibiotics may elicit metabolic disorder,alter protein expression,and then induce pathological changes in zebrafish embryos,despite negligible physiological and developmental toxicity.This study investigated the single and combined developmental toxicity of fluoroquinolones(enrofloxacin[ENR]and ciprofloxacin[CIP])(
0.5 mM)and heavy metals(Cu and Cd)(
0.5 mM)to zebrafish embryos,and molecular responses of zebrafish larvae upon exposure to the single pollutant(0.2 mM)or a binary metal-fluoroquinolone mixture(0.2 mM).In all single and mixture exposure groups,no developmental toxicity was observed,but oxidative stress,inflammation,and lipid depletion were found in zebrafish embryos,which was more severe in the mixture exposure groups than in the single exposure groups,probably due to increased metal bioaccumulation in the presence of ENR or CIP.Metabolomics analysis revealed the up-regulation of amino acids and down-regulation of fatty acids,corresponding to an active response to oxidative stress and the occurrence of inflammation.The up-regulation of antioxidase and immune proteins revealed by proteomics analysis further confirmed the occurrence of oxidative stress and inflammation.Furthermore,the KEGG pathway enrichment analysis showed a significant disturbance of pathways related to immunity and tumor,indicating the potential risk of tumorigenesis in zebrafish larvae.The findings provide molecular-level insights into the adverse effects of heavy metals and antibiotics(especially in chemical mixtures)on zebrafish embryos,and highlight the potential ecotoxicological risks of trace-level heavy metals and antibiotics in the environment.

关 键 词：FLUOROQUINOLONE Metal INFLAMMATION Tumor Oxidative stress 

分 类 号：Q78[生物学—分子生物学]