Anesthesia protocol for ear surgery in Wistar rats(animal research)  

作　　者：Abhijeet Bhatia Pranjal Pratim Saikia Barilin Dkhar Haphidasara Pyngrope 

机构地区：[1]Department of ENT,North Eastern Indira Gandhi Regional Institute of Health&Medical Sciences(NEIGRIHMS),Shillong,India [2]Department of Pharmacology(Animal House),North Eastern Indira Gandhi Regional Institute of Health&Medical Sciences(NEIGRIHMS),Shillong,India

出　　处：《Animal Models and Experimental Medicine》2022年第2期183-188,共6页动物模型与实验医学（英文）

基　　金：The study was a part of the research project funded by the Department of Biotechnology,Ministry of Science and Technology,Government of India(project no.BMB/2015/56)and DBT Research Associateship program(2018)。

摘　　要：Objective:To formulate an anesthesia protocol for safe and satisfactory anesthesia for ear surgery in rats.Methods:The rats were anesthetized with xylazine(10 mg/kg body weight)and keta-mine at doses of 80,50,40,and 30 mg/kg body weight or with isoflurane anesthesia(2%-3.5%in 100%oxygen;maintenance dose 1.5%-3.5%).The anesthesia induction,surgery,and recovery time were recorded.Results:In total,17 rats were induced by varying doses of ketamine-xylazine and 28 rats with isoflurane.Mean induction time with ketamine-xylazine was 6±2.9 min compared with 3.8±1.1 min with isoflurane.Mean recovery time with ketamine-xylazine was 142.6±49.3 min compared with 4.1±1.2 min with isoflurane.A mortal-ity of 4 animals after developing dyspnea was recorded with ketamine-xylazine.Conclusion:Isoflurane anesthesia offers appropriate induction and recovery times and low mortality rates for the surgeries performed.Isoflurane anesthesia offers reli-able results for ear surgery in rats.However,more equipment and technical skills are needed.

关 键 词：ISOFLURANE KETAMINE OTOLOGY Wistar rat XYLAZINE 

分 类 号：R614[医药卫生—麻醉学]