亚慢性经口染毒纳米三氧化二铁致大鼠免疫毒性的作用  

Immunotoxicity of subchronic oral exposure of iron oxide nanoparticles in rats

作　　者：邵黄芳 刘洋[1] 王雪 王丹 任冬霞[1] 李育林 李云[1] Shao Huangfang;Liu Yang;Wang Xue;Wang Dan;Ren Dongxia;Li Yulin;Li Yun(West China School of Public Health/Sichuan University West China Fourth Hospital,Sichuan University,Chengdu 610041,China;Yaohai District Center for Disease Control and Prevention,Hefei 230011,China)

机构地区：[1]四川大学华西公共卫生学院/四川大学华西第四医院,成都610041 [2]合肥市瑶海区疾病预防控制中心,合肥230011

出　　处：《卫生研究》2022年第2期266-270,292,共6页Journal of Hygiene Research

基　　金：国家重点研发计划(No.2017YFC1600204)。

摘　　要：目的初步探讨纳米三氧化二铁(iron oxide nanoparticles,Fe_(2)O_(3)NPs)亚慢性经口暴露后对大鼠的免疫毒性效应及其机制。方法将40只雌性SD大鼠按体重随机分为溶剂对照组(0.5%羟丙基甲基纤维素),70 nm Fe_(2)O_(3)NPs低(50 mg/kg)、中(100 mg/kg)、高(200 mg/kg)剂量组,连续灌胃90天。观察大鼠一般状况和体重变化,试验结束后,腹主动脉放血处死,摘取脾脏和胸腺,称重并计算脏器系数,苏木精-伊红染色法检查组织病理学改变,流式细胞仪法测定脾脏T淋巴细胞亚群,试剂盒法检测脾脏、胸腺中丙二醛(malondialdehyde,MDA)含量、超氧化物歧化酶(superoxide dismutase,SOD)活性、谷胱甘肽(glutathione,GSH)含量和血清免疫球蛋白A(immunoglobulin A,IgA)、M(IgM)、G(IgG)以及肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、γ干扰素(gamma interferon,IFN-γ)含量。结果与溶剂对照组相比,各染毒组大鼠的一般状态、体重、脾脏和胸腺系数及组织结构、脾脏T淋巴细胞亚群及血清免疫球蛋白均未发现明显改变;低、中剂量染毒组大鼠脾脏MDA含量升高,SOD活性降低,高剂量组GSH含量降低(P<0.05);各染毒组大鼠胸腺MDA含量升高,SOD活性下降(P<0.05),GSH含量差异无统计学意义;各染毒组大鼠血清TNF-α含量下降(P<0.05),IFN-γ含量差异无统计学意义。结论在本实验条件下,亚慢性经口暴露纳米三氧化二铁可引起大鼠主要免疫器官脾脏和胸腺的氧化损伤,并可对血清细胞因子产生影响,毒性效应主要与氧化应激有关。OBJECTIVE To investigate the immunotoxic effects and its mechanism of iron oxide nanoparticles(Fe_(2)O_(3)NPs)on rats after subchronic oral exposure.METHODS Forty female SD rats were randomly divided into solvent control(0.5%hydroxypropyl methylcellulose),70 nm Fe_(2)O_(3)NPs low(50 mg/kg),medium(100 mg/kg)and high(200 mg/kg)dose groups according to their body weight.The rats were observed for general condition and body weight changes,gavaged for 90 days,and executed by bleeding from the abdominal aorta.At the end of the test,the spleen and thymus were removed,weighed and organ coefficients were calculated,histopathological changes were examined by hematoxylin and eosin(HE)staining,T-lymphocyte subsets in the spleen were determined by flow cytometry,and the malondialdehyde(MDA)content,superoxide dismutase(SOD)activity,glutathione(GSH)content in spleen and thymus and immunoglobulin A(IgA),M(IgM)and G(IgG)as well as tumor necrosis factor-α(TNF-α)and gamma interferon(IFN-γ)content in serum were measured by the kit.RESULTS Compared with the solvent control group:no significant changes were found in the general status,body weight,spleen and thymus coefficients and tissue structure,splenic T-lymphocyte subpopulation and serum immunoglobulins of the rats in each treatment group;The spleen MDA content was increased and SOD activity was decreased in the low and medium dose groups of rats,and the GSH content was decreased in the high dose group(all P<0.05);The thymus MDA content was increased and SOD activity was decreased in each treatment group(all P<0.05),and the difference in GSH content was not statistically significant(P>0.05);The serum TNF-αcontent of rats in all the treatment groups decreased(P<0.05),and the difference in IFN-γcontent was not statistically significant(P>0.05).CONCLUSION Under the experimental conditions,subchronic oral exposure of Fe_(2)O_(3)NPs can cause oxidative damage to the main immune organs(spleen and thymus)of rats,and can affect serum cytokines,and the toxic effects are mainly related

关 键 词：纳米材料 纳米FE O 食品卫生 免疫毒性 

分 类 号：R155.5[医药卫生—营养与食品卫生学]