运用多重PCR技术建立一种田螺的基因检测方法  

Multiplex PCR Technology was used to Establish a Genetic Detection Method for Cipangopaludina chinensis Gray

作　　者：胡江如 蒋艳 韩素素 胡爽 徐帅磊 粟金岭 陈正培 HU Jiangru;JIANG Yan;HAN Susu;HU Shuang;XU Shuailei;SU Jinling;CHEN Zhengpei(Tendering and Purchasing Center,Liuzhou Institute of Technology,Liuzhou 545616,China;Department of Food and Chemical Engineering,Liuzhou Institute of Technology,Liuzhou 545616,China;Department of Mechanical Engineering,Liuzhou Institute of Technology,Liuzhou 545616,China)

机构地区：[1]柳州工学院招标采购中心,广西柳州545616 [2]柳州工学院食品与化学工程学院,广西柳州545616 [3]柳州工学院机械工程学院,广西柳州545616

出　　处：《现代食品》2022年第7期195-197,201,共4页Modern Food

基　　金：广西中青年教师科研能力提升项目(2019KY1086);大学生创新创业训练项目(201913639020)。

摘　　要：田螺是柳州螺蛳粉高汤熬制的必备原料,为了鉴别汤料真伪,本研究基于PCR技术建立了一种田螺成分的检测方法,并对该方法的特异性进行测试。结果显示,在引物为F4/R4和F4/R5,退火温度为60℃的条件下,检测效果较好。通过田螺检测方法的建立,为后续鉴定螺蛳粉汤真伪提供技术支持。Field snail is an essential raw material for boiling Liuzhou Cipangopaludina chinensis Gray broth.In order to identify the authenticity of the soup,a method for detecting snail composition was established based on PCR technology,and the specificity of this method was tested.The detection effect is better under the conditions of F4/R4 and F4/R5,and the annealing temperature is 60℃.The establishment of the field screw detection method,which provided technical support for the subsequent identification of the authenticity of snail powder decoction.

关 键 词：多重PCR技术 田螺 基因检测 

分 类 号：TS264.2[轻工技术与工程—发酵工程]