微生物固态发酵工艺对红薯淀粉渣真蛋白含量的影响  

Effect of Microbial Solid State Fermentation Process on The True Protein Content of Sweet Potato Starch Residue

作　　者：王妍 郑猛虎 崔欣雨 韦子海 吴冠中 徐春城[1] WANG Yan;ZHENG Menghu;CUI Xinyu;WEI Zihai;WU Guanzhong;XU Chuncheng(College of Engineering,China Agricultural University,Beijing 100083,China;Ningbo Ningxing Yongyou Feed Co.,Ltd.,Zhejiang Ningbo 315311,China)

机构地区：[1]中国农业大学工学院,北京100083 [2]宁波宁兴涌优饲料有限公司,浙江宁波315311

出　　处：《饲料工业》2022年第9期35-40,共6页Feed Industry

基　　金：宁波市慈溪市农业农村局:农作物秸秆、农副产品饲料化关键技术研究与示范项目[CN201902]。

摘　　要：研究旨在优化微生物固态发酵提高红薯淀粉渣真蛋白含量的工艺。以红薯淀粉渣为主要原料,利用14种不同类型菌株进行单因素发酵试验,并利用响应面法优化发酵条件。在不同类型的微生物中,米曲霉发酵后产物的真蛋白含量最高;最佳的发酵菌种组合为米曲霉+巨大芽孢杆菌+库德里阿兹威氏毕赤酵母,接种比例为3:2:1,接种量为1×10^(6)CFU/g DM;响应面法优化的最佳发酵条件(温度33℃、初始含水量66%、时间86 h)下,红薯淀粉渣发酵饲料真蛋白含量为12.11%,约为未发酵底物的3倍,接近模型预测值。复合微生物固态发酵是提高红薯淀粉渣发酵饲料真蛋白含量的有效方法。This stucly aimed to optimize the process of microbial solid-state fermentation to increase the true protein content of sweet potato starch residue.Sweet potato residue was used as the main substrates.Fourteen different microbial strains were evaluated for their ability to produce true protein.The effects of strains,strain combinations,inoculation ratio and inoculation amount on the true protein content of sweet potato starch residue fermentation products were analyzed through single factor experiments,and the fermentation conditions were optimized by response surface methodology.Among different types of microorganisms,the product of Aspergillus oryzae fermentation has the highest true protein content;The ideal strain combination was Aspergillus oryzae+Bacillus megaterium+Picha kudriavzevii,the inoculation ratio was 3:2:1,and the inoculation amount was 1×106CFU/g DM;The optimal fermentation conditions optimized by response surface methodology were fermentation temperature of 33℃,initial water content of66%,fermentation time of 86 h.The true protein content of sweet potato starch residue by microbial solid-state fermentation was 12.11%under these conditions,which was 3 times of that in the unfermented substrates,and was close to the predicted value of the model.Composite microbial solid-state fermentation was an effective method to increase the true protein content of fermented sweet potato starch residues.

关 键 词：红薯淀粉渣 固态发酵 菌种组合 发酵条件 响应面优化 

分 类 号：S816.6[农业科学—饲料科学]