改性红枣多糖的制备和结构表征  

Preparation and Structure Characterization of Modified Jujube Polysaccharides

作　　者：杨燕敏 郑振佳[1] 李桂芹[2] 张仁堂[1] YANG Yan-min;ZHENG Zhen-jia;LI Gui-qin;ZHANG Ren-tang(College of Food Science and Engineering,Shandong Agricultural University,Key Laboratory of Food Processing Technology and Quality Control in Shandong Province,Taian 271018,Shandong,China;Department of Biological Engineering,Binzhou Vocational College,Binzhou 256603,Shandong,China)

机构地区：[1]山东农业大学食品科学与工程学院,山东省高校食品加工技术与质量控制重点实验室,山东泰安271018 [2]滨州职业学院生物工程学院,山东滨州256603

出　　处：《食品研究与开发》2022年第9期8-14,共7页Food Research and Development

基　　金：中央引导地方科技发展资金项目(YDZX2021071);2021年度山东省重点研发计划项目(2021TZXD011);德州市健康食品产业创新创业共同体;山东省土地发展集团有限公司科技项目。

摘　　要：以哈密大枣为原料,采用水提醇沉法提取和脱脂脱蛋白透析获得红枣多糖,通过羧甲基化法、硫酸法和硒化法对红枣多糖进行改性,并对其改性后的红枣多糖进行初步结构表征。结果表明:红枣多糖分子量为136413 Da、多糖含量为49.96%、;羧甲基化红枣多糖的得率最高为91.1%,取代度为0.42、多糖含量为36.51%、分子量为46637 Da;硫酸化红枣多糖的取代度为1.65、多糖含量为42.73%、分子量为9970 Da和6149 Da;硒化红枣多糖硒元素含量为(580.15±11.96)μg/g、多糖含量为44.56%、分子量为136153 Da。单糖组成分析中硒化红枣多糖半乳糖醛酸含量最高,为53.23%,硫酸化红枣多糖、羧甲基化红枣多糖与红枣多糖中半乳糖醛酸含量(48.17%)相比均有所降低,分别为30.51%和39.60%。红外光谱分析的表征结果可知改性后多糖仍具有多糖的官能团,其他红外指标的变化说明改性成功。Jujube polysaccharide was obtained by water-extraction and alcohol-precipitation method from Ziziphus jujuba cv.Hamidazao and purified by defatting,deproteinization,and dialysis.The purified jujube polysaccharide was respectively modified by carboxymethylation,sulfation,and selenization,and then the structures of modified jujube polysaccharides were characterized.The jujube polysaccharide had a molecular weight of 136413 Da and the polysaccharide content of 49.96%.The carboxymethylated jujube polysaccharide had the highest yield(91.1%),with the substitution degree of 0.42,the polysaccharide content of 36.51%,and the molecular weight of 46637 Da.The sulfated jujube polysaccharide had the substitution degree of 1.65,the polysaccharide content of 42.73%,and the molecular weights of 9970 Da and 6149 Da.The selenized jujube polysaccharide had the selenium content of(580.15±11.96)μg/g,the polysaccharide content of 44.56%,and the molecular weight of 136153 Da.The selenized jujube polysaccharide had the highest galacturonic acid content(53.23%).The content of galacturonic acid in jujube polysaccharide(48.17%)was higher than that in sulfated jujube polysaccharide(30.51%)and carboxymethylated jujube polysaccharide(39.60%).The infrared spectra showed that the modified polysaccharides still had functional groups of polysaccharides,and the change of other infrared indicators indicated that the modification was successful.

关 键 词：红枣多糖 硫酸改性 羧甲基改性 硒化改性 结构表征 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]