黑果腺肋花楸果微乳系统薄层色谱分析及生物自显影抗氧化活性研究  

Analysis of microemulsion system using thin-layer chromatographic in black chokeberry and its bioautography for screening antioxidant components

作　　者：张婷婷[1] 摆富叶 李乔乔 李兰兰 安泽冲 加尼赛力克·巴特尔汗 李新霞[1] ZHANG Tingting;BAI Fuye;LI Qiaoqiao;LI Lanlan;AN Zechong;JIANISAILIKE·Bateerhan;LI Xinxia(College of Pharmacy,Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830011

出　　处：《食品与发酵工业》2022年第9期139-145,共7页Food and Fermentation Industries

基　　金：2021年新疆维吾尔自治区重大科技专项(2021A03002-2)。

摘　　要：建立了新食品原料黑果腺肋花楸果实中多种成分半定量测定方法,并测定其抗氧化能力及初步确定抗氧化活性成分。以质量分数75%含水量微乳-甲酸(体积比9∶1)为展开剂,采用高效薄层色谱法同时对黑果腺肋花楸果实中矢车菊素-3-O-葡萄糖苷、金丝桃苷和绿原酸进行含量测定,并采用DPPH自由基法和薄层生物自显影技术初步确定黑果腺肋花楸果中抗氧化活性成分。结果表明,方法学考察表明微乳系统薄层色谱分析方法良好;维生素C清除50%DPPH自由基质量浓度约为13.5μg/mL,黑果腺肋花楸质量浓度约为0.18 mg/mL;薄层生物自显影结果表明,黑果腺肋花楸果中矢车菊素-3-O-阿拉伯糖苷、矢车菊素-3-O-葡萄糖苷、金丝桃苷、绿原酸,新绿原酸均具抗氧化活性。该研究建立的高效薄层扫描方法重复性、稳定性好,可作为一个简单、快速的半定量方法,黑果腺肋花楸果中抗氧化活性的测定及成分指认可为后续研究奠定基础。A semi-quantitative method was established for the determination of various components in black chokeberry,and its antioxidant capacity and antioxidant active components were determined.High-performance thin-layer chromatography was used to analyze the cyanidin-3-O-glucoside,hyperoside and chlorogenic acid of black chokeberry simultaneously,with 75% microemulsion-formic acid(9:1) as the eluent.The methods of DPPH free radicals and thin layer chromatography(TLC)-bioautography technique were used to determine its antioxidant capacity of black chokeberry.The results showed that the TLC analysis method was good for the detection of microemulsion system.The determination of DPPH scavenging ability showed that the IC_(50) of vitamin C was 13.5 μg/mL,while black chokeberry was 0.18 mg/mL.TLC-bioautography results showed that cyanidin-3-O-glucoside,cyanidin-3-O-arabinoside,hyperoside,chlorogenic acid and neochlorogenic acid,all of which had antioxidant activity.The established high-efficiency thin-layer scanning method was reproducible,stable and could be used as a simple and rapid semi-quantitative method to determine the content of chemical components in black chokeberry.The determination of antioxidant activity and the recognition of the composition of black chokeberry provides the basis for follow-up studies.

关 键 词：黑果腺肋花楸果 多成分 微乳系统薄层色谱 生物自显影 抗氧化活性 

分 类 号：TS201[轻工技术与工程—食品科学] O657.7[轻工技术与工程—食品科学与工程]