Reduced graphene oxide-embedded nerve conduits loaded with bone marrow mesenchymal stem cell-derived extracellular vesicles promote peripheral nerve regeneration  

作　　者：Wei Zhang Xing-Xing Fang Qi-Cheng Li Wei Pi Na Han 

机构地区：[1]Department of Orthopedics and Trauma,Peking University People’s Hospital,Beijing,China [2]National Center for Trauma Medicine,Beijing,China [3]Central Laboratory,Peking University People’s Hospital,Beijing,China

出　　处：《Neural Regeneration Research》2023年第1期200-206,共7页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China, No. 31671248;the Natural Science Foundation of Beijing, No. 7222198 (both to NH)

摘　　要：We previously combined reduced graphene oxide(rGO)with gelatin-methacryloyl(GelMA)and polycaprolactone(PCL)to create an rGO-GelMA-PCL nerve conduit and found that the conductivity and biocompatibility were improved.However,the rGO-GelMA-PCL nerve conduits differed greatly from autologous nerve transplants in their ability to promote the regeneration of injured peripheral nerves and axonal sprouting.Extracellular vesicles derived from bone marrow mesenchymal stem cells(BMSCs)can be loaded into rGO-GelMA-PCL nerve conduits for repair of rat sciatic nerve injury because they can promote angiogenesis at the injured site.In this study,12 weeks after surgery,sciatic nerve function was measured by electrophysiology and sciatic nerve function index,and myelin sheath and axon regeneration were observed by electron microscopy,immunohistochemistry,and immunofluorescence.The regeneration of microvessel was observed by immunofluorescence.Our results showed that rGO-GelMA-PCL nerve conduits loaded with BMSC-derived extracellular vesicles were superior to rGO-GelMA-PCL conduits alone in their ability to increase the number of newly formed vessels and axonal sprouts at the injury site as well as the recovery of neurological function.These findings indicate that rGO-GelMA-PCL nerve conduits loaded with BMSC-derived extracellular vesicles can promote peripheral nerve regeneration and neurological function recovery,and provide a new direction for the curation of peripheral nerve defect in the clinic.

关 键 词：ANGIOGENESIS AXON bone mesenchymal stem cell extracellular vesicles hybrid nanofibers myelin sheath nerve conduit neurological function peripheral nerve injury reduced graphene oxide 

分 类 号：R651.2[医药卫生—外科学]